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Herein, we report a BACE1\u2010disassemblable nanoprobe,\n                    <jats:bold>\n                      <jats:sup>19<\/jats:sup>\n                      F\u2010Cy5.5\u2010NP\n                    <\/jats:bold>\n                    for \u201cTurn\u2010On\u201d\n                    <jats:sup>19<\/jats:sup>\n                    F magnetic resonance\/near\u2010infrared fluorescence (\n                    <jats:sup>19<\/jats:sup>\n                    F MR\/NIR\u2010FL) imaging of the enzyme activity in AD in vivo. The \u201cTurn\u2010On\u201d\n                    <jats:sup>19<\/jats:sup>\n                    F MRI signals are employed for imaging BACE1 activity with high specificity, while the \u201cTurn\u2010On\u201d NIR\u2010FL signals are used for double checking the enzyme activity with high spatial resolution. Specifically,\n                    <jats:bold>\n                      <jats:sup>19<\/jats:sup>\n                      F\u2010Cy5.5\u2010NP,\n                    <\/jats:bold>\n                    with a silent\n                    <jats:sup>19<\/jats:sup>\n                    F MR\/NIR\u2010FL signal is obtained from the precursor Cys(StBu)\u2010Glu\u2010Val\u2010Asn\u2010Leu\u2010Asp\u2010Ala\u2010Glu\u2010Phe(CF\n                    <jats:sub>3<\/jats:sub>\n                    )\u2010Lys(Cy5.5)\u2010CBT (\n                    <jats:bold>\n                      <jats:sup>19<\/jats:sup>\n                      F\u2010Cy5.5\n                    <\/jats:bold>\n                    ) through a CBT\u2010Cys click reaction. Upon BACE1 cleavage, the nanoprobe disassembles, resulting in 6.8 \u00b1 1.3\u2010fold and 9.5 \u00b1 0.3\u2010fold increases of\n                    <jats:sup>1<\/jats:sup>\n                    <jats:sup>9<\/jats:sup>\n                    F NMR and NIR\u2010FL signals in vitro, respectively. Moreover,\n                    <jats:bold>\n                      <jats:sup>19<\/jats:sup>\n                      F\u2010Cy5.5\u2010NP\n                    <\/jats:bold>\n                    renders 4.6 \u00b1 0.9\u2010fold\/1.5 \u00b1 0.1\u2010fold higher\n                    <jats:sup>19<\/jats:sup>\n                    F MRI\/NIR\u2010FL signal in A\u03b2\n                    <jats:sub>25\u201335<\/jats:sub>\n                    \u2010treated PC12 cells than that in normal PC12 cells. In vivo experimental results show that this nanoprobe enables the precise imaging of BACE1 activity in AD zebrafish models. We anticipate that\n                    <jats:bold>\n                      <jats:sup>19<\/jats:sup>\n                      F\u2010Cy5.5\u2010NP\n                    <\/jats:bold>\n                    could be applied for the early diagnosis of AD in clinics in the future.\n                  <\/jats:p>","DOI":"10.1002\/anie.1325073","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:54:58Z","timestamp":1780966498000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A\n                    <sup>19<\/sup>\n                    F MRI\/NIR\u2010FL Nanoprobe for \u201cTurn\u2010On\u201d Imaging of \u03b2\u2010Secretase (BACE1) Activity in Alzheimer's Disease"],"prefix":"10.1002","volume":"65","author":[{"given":"Lingling","family":"Xu","sequence":"first","affiliation":[{"name":"School of Chemistry and Materials Yangzhou University  Yangzhou 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Engineering Southeast University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010Dong","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Digital Medical Engineering School of Biological Science and Medical Engineering Southeast University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Digital Medical Engineering School of Biological Science and Medical Engineering Southeast University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanjun","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Materials Yangzhou University  Yangzhou 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