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Here we introduce a one\u2010pot strategy that couples aqueous ring\u2010opening polymerization\u2010induced self\u2010assembly (ROPISA) of \u03b3\u2010propargyl\u2010L\u2010glutamate\n                    <jats:italic>N<\/jats:italic>\n                    \u2010carboxyanhydride with copper\u2010catalyzed azide\u2013alkyne cycloaddition (CuAAC) to produce fluorescent polypeptide nanoparticles. This one\u2010pot \u201cClick'n ROPISA\u201d process enables the simultaneous formation of polypeptide nano\u2010objects and covalent incorporation of diverse azido\u2010functionalized fluorophores. The resulting assemblies exhibit tunable emission across the visible spectrum and are accessible at high solids content while maintaining high brightness and structural uniformity. Dense fluorophore incorporation enables efficient F\u00f6rster resonance energy transfer (FRET) both within the assemblies and with dye\u2010labeled proteins, supporting sensing applications. The intense brightness of the red\u2010emitting nanoparticles further enables single\u2010particle tracking in microscopy toward advanced bioimaging.\n                  <\/jats:p>","DOI":"10.1002\/anie.1373955","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T12:31:04Z","timestamp":1784118664000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Click'n ROPISA: One\u2010Pot Synthesis of Ultrabright Fluorescent Nanoparticles Made of Polypeptides"],"prefix":"10.1002","volume":"65","author":[{"given":"Amin El Jarroudi","family":"Hammou","sequence":"first","affiliation":[{"name":"University of Bordeaux CNRS Bordeaux INP ISM UMR 5255  Talence France"},{"name":"University of Bordeaux CNRS Bordeaux INP LCPO UMR 5629  Pessac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliette","family":"Lavainne","sequence":"additional","affiliation":[{"name":"University of 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