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We successfully incorporated five different metals (Ga, In, Tb, Lu, and Y) along designed oligonucleotide templates, including the simultaneous incorporation of two different metal chelates within a given strand. To provide bio\u2010orthogonality, this approach exploits the well\u2010known enzymatic fidelity of DNA polymerase to generate uniformly metal\u2010loaded DNA constructs with well\u2010defined and programmable compositions. The platform is validated using chelators DOTA and DTPA and confirmed by both inductively coupled plasma mass spectrometry (ICP\u2010MS) quantification and autoradiographic analysis following gel electrophoresis to demonstrate radioisotope incorporation with clinically relevant metals (\n                    <jats:sup>68<\/jats:sup>\n                    Ga for PET,\n                    <jats:sup>111<\/jats:sup>\n                    In for SPECT,\n                    <jats:sup>161<\/jats:sup>\n                    Tb and\n                    <jats:sup>177<\/jats:sup>\n                    Lu for \u03b2\n                    <jats:sup>\u2212<\/jats:sup>\n                    therapy), establishing the potential for using the information content of DNA to create multi\u2010metalated radiopharmaceuticals. This programmable platform establishes a new paradigm for metalated molecular probe development with versatility for potential therapeutic, theranostic, and mass spectrometry\u2010based applications, and potentially generalizable to other metal isotopes based on diagnostic or therapeutic requirements.\n                  <\/jats:p>","DOI":"10.1002\/anie.1429706","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T10:29:12Z","timestamp":1778149752000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Toward Multi(radio)metalated DNA: Enzymatic Polymerization of Metal\u2010Chelate\u2010Modified Deoxyribonucleoside Triphosphates"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4617-9248","authenticated-orcid":false,"given":"Antonio A. W. 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