{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:01:05Z","timestamp":1791234065273,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"46","license":[{"start":{"date-parts":[[2005,10,25]],"date-time":"2005-10-25T00:00:00Z","timestamp":1130198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2005,11,25]]},"DOI":"10.1002\/anie.200501552","type":"journal-article","created":{"date-parts":[[2005,10,25]],"date-time":"2005-10-25T09:49:28Z","timestamp":1130233768000},"page":"7612-7615","source":"Crossref","is-referenced-by-count":124,"title":["Quantum Dots as Efficient Energy Acceptors in a Time\u2010Resolved Fluoroimmunoassay"],"prefix":"10.1002","volume":"44","author":[{"given":"Niko","family":"Hildebrandt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc J.","family":"Charbonni\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Beck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond F.","family":"Ziessel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans\u2010Gerd","family":"L\u00f6hmannsr\u00f6ben","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,21]]},"reference":[{"key":"e_1_2_4_1_2","unstructured":"\u00a0"},{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5251.933"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00072a025"},{"key":"e_1_2_4_4_2","unstructured":"\u00a0"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja970754m"},{"key":"e_1_2_4_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/nl025596y"},{"key":"e_1_2_4_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077194"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5385.2016"},{"key":"e_1_2_4_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104274"},{"key":"e_1_2_4_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200301758"},{"key":"e_1_2_4_10_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200301758"},{"key":"e_1_2_4_11_2","unstructured":"\u00a0"},{"key":"e_1_2_4_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200400651"},{"key":"e_1_2_4_12_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200400651"},{"key":"e_1_2_4_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja037088b"},{"key":"e_1_2_4_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200461491"},{"key":"e_1_2_4_14_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200461491"},{"key":"e_1_2_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat961"},{"key":"e_1_2_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02571"},{"key":"e_1_2_4_17_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.76.1517"},{"key":"e_1_2_4_18_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.1814795"},{"key":"e_1_2_4_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja045676z"},{"key":"e_1_2_4_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/nl015519n"},{"key":"e_1_2_4_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja031886k"},{"key":"e_1_2_4_22_2","unstructured":"The choice of the buffering medium was guided by the use of this buffer for optimal photo\u2010physical properties of qdots. Changing to PBS buffer resulted in significant losses in energy\u2010transfer efficiency."},{"key":"e_1_2_4_23_2","unstructured":"Qdot Biotin Conjugates User Manual (Cat. # 1030\u20101 Cat. # 1032\u20101) http:\/\/www.qdots.com."},{"key":"e_1_2_4_24_2","unstructured":"\u00a0"},{"key":"e_1_2_4_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp035756"},{"key":"e_1_2_4_26_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.88.137401"},{"key":"e_1_2_4_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/andp.19484370105"},{"key":"e_1_2_4_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-3061-6"},{"key":"e_1_2_4_29_2","volume-title":"Molecular Fluorescence, Principles and Applications","author":"Valeur B.","year":"2002"},{"key":"e_1_2_4_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja003699h"},{"key":"e_1_2_4_31_2","unstructured":"Preliminary studies on Tb excited\u2010state lifetimes have shown that introduction of BSA in the buffer plays a significant role. Nonspecific binding interactions of BSA and streptavidin that are able to compete with biotin\u2010streptavidin recognition have previously been reported.[23]In our case this nonspecific binding results in slow equilibration of the system. The interaction of BSA with streptavidin must first be disrupted before the thermodynamically favorable TbL\u2010Strep\/Biot\u2010QD interaction can take place. Peeling off the BSA from the Tb emitting species eliminates the associated quenching processes and is probably the origin of the rising Tb emission for [Biot\u2010QD]\/[TbL\u2010Strep]<0.25 (see Supporting Information)."},{"key":"e_1_2_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(98)00083-1"},{"key":"e_1_2_4_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20129"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fanie.200501552","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.200501552","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T06:55:49Z","timestamp":1697007349000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.200501552"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11,21]]},"references-count":36,"journal-issue":{"issue":"46","published-print":{"date-parts":[[2005,11,25]]}},"alternative-id":["10.1002\/anie.200501552"],"URL":"https:\/\/doi.org\/10.1002\/anie.200501552","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,11,21]]}}}