{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:34:04Z","timestamp":1791272044583,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,12,9]],"date-time":"2013-12-09T00:00:00Z","timestamp":1386547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Australian National Health and Medical Research Council (NHMRC)","award":["569927"],"award-info":[{"award-number":["569927"]}]},{"name":"Australian National Health and Medical Research Council (NHMRC)","award":["10123388"],"award-info":[{"award-number":["10123388"]}]},{"DOI":"10.13039\/501100001794","name":"The University of Queensland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001794","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2014,1,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mambalgins are a novel class of snake venom components that exert potent analgesic effects mediated through the inhibition of acid\u2010sensing ion channels (ASICs). The 57\u2010residue polypeptide mambalgin\u20102 (Ma\u20102) was synthesized by using a combination of solid\u2010phase peptide synthesis and native chemical ligation. The structure of the synthetic toxin, determined using homonuclear NMR, revealed an unusual three\u2010finger toxin fold reminiscent of functionally unrelated snake toxins. Electrophysiological analysis of Ma\u20102 on wild\u2010type and mutant ASIC1a receptors allowed us to identify \u03b1\u2010helix 5, which borders on the functionally critical acidic pocket of the channel, as a major part of the Ma\u20102 binding site. This region is also crucial for the interaction of ASIC1a with the spider toxin PcTx1, thus suggesting that the binding sites for these toxins substantially overlap. This work lays the foundation for structure\u2013activity relationship (SAR) studies and further development of this promising analgesic peptide.<\/jats:p>","DOI":"10.1002\/anie.201308898","type":"journal-article","created":{"date-parts":[[2013,12,9]],"date-time":"2013-12-09T08:36:57Z","timestamp":1386578217000},"page":"1017-1020","source":"Crossref","is-referenced-by-count":70,"title":["Chemical Synthesis, 3D Structure, and ASIC Binding Site of the Toxin Mambalgin\u20102"],"prefix":"10.1002","volume":"53","author":[{"given":"Christina I.","family":"Schroeder","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lachlan D.","family":"Rash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Vila\u2010Farr\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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