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Here we applied site\u2010specific bioorthogonal labeling on anthrax lethal factor (LF) to visualize its membrane translocation inside live cells. In contrast to the previously reported FP tags that significantly perturbed LF\u2019s membrane trafficking, our precisely and quantitatively labeled LF exhibited an endocytic activity comparable to wild\u2010type LF. This allowed time\u2010lapse imaging of LF\u2019s natural translocation process from host cell membrane to cytosol, which revealed molecular details of its virulence mechanism. Our strategy is generally applicable for monitoring intracellular protein membrane translocation that is difficult to access using conventional protein labeling methodologies.<\/jats:p>","DOI":"10.1002\/anie.201403945","type":"journal-article","created":{"date-parts":[[2014,5,14]],"date-time":"2014-05-14T19:29:11Z","timestamp":1400095751000},"page":"6449-6453","source":"Crossref","is-referenced-by-count":20,"title":["Monitoring Endocytic Trafficking of Anthrax Lethal Factor by Precise and Quantitative Protein Labeling"],"prefix":"10.1002","volume":"53","author":[{"given":"Siqi","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng 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