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Inhibiting these multivalent protein\/carbohydrate interactions would prevent the toxin from entering cells and causing diarrhea. Here we demonstrate that the site\u2010specific modification of a protein scaffold, which is perfectly matched in both size and valency to the target toxin, provides a convenient route to an effective multivalent inhibitor. The resulting pentavalent neoglycoprotein displays an inhibition potency (IC<jats:sub>50<\/jats:sub>) of 104\u2005p<jats:sc>M<\/jats:sc> for the CT B\u2010subunit (CTB), which is the most potent pentavalent inhibitor for this target reported thus far. Complexation of the inhibitor and CTB resulted in a protein heterodimer. This inhibition strategy can potentially be applied to many multivalent receptors and also opens up new possibilities for protein assembly strategies.<\/jats:p>","DOI":"10.1002\/anie.201404397","type":"journal-article","created":{"date-parts":[[2014,7,2]],"date-time":"2014-07-02T21:05:28Z","timestamp":1404335128000},"page":"8323-8327","source":"Crossref","is-referenced-by-count":62,"title":["A Protein\u2010Based Pentavalent Inhibitor of the Cholera Toxin B\u2010Subunit"],"prefix":"10.1002","volume":"53","author":[{"given":"Thomas R.","family":"Branson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom E.","family":"McAllister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Garcia\u2010Hartjes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin A.","family":"Fascione","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James F.","family":"Ross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart L.","family":"Warriner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Wennekes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Zuilhof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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