{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:55:23Z","timestamp":1789952123189,"version":"4.0.1"},"reference-count":42,"publisher":"Wiley","issue":"42","license":[{"start":{"date-parts":[[2014,8,27]],"date-time":"2014-08-27T00:00:00Z","timestamp":1409097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2014,10,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Polyketide synthases (PKSs) usually employ a ketoreductase (KR) to catalyze the reduction of a \u03b2\u2010keto group, followed by a dehydratase (DH) that drives the dehydration to form a double bond between the \u03b1\u2010 and \u03b2\u2010carbon atoms. Herein, a DH*\u2010KR* involved in FR901464 biosynthesis was characterized: DH* acts on glyceryl\u2010S\u2010acyl carrier protein (ACP) to yield ACP\u2010linked pyruvate; subsequently KR* reduces \u03b1\u2010ketone that yields\n                    <jats:sc>L<\/jats:sc>\n                    \u2010lactyl\u2010S\u2010ACP as starter unit for polyketide biosynthesis. Genetic and biochemical evidence was found to support a similar pathway that is involved in the biosynthesis of lankacidins. These results not only identified new PKS domains acting on different substrates, but also provided additional options for engineering the PKS starter pathway or biocatalysis.\n                  <\/jats:p>","DOI":"10.1002\/anie.201406602","type":"journal-article","created":{"date-parts":[[2014,8,27]],"date-time":"2014-08-27T06:42:52Z","timestamp":1409121772000},"page":"11315-11319","source":"Crossref","is-referenced-by-count":23,"title":["An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing \u03b1\u2010Ketone in Polyketide Starter Unit Biosynthesis"],"prefix":"10.1002","volume":"53","author":[{"given":"Hai\u2010Yan","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man\u2010Cheng","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gong\u2010Li","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,27]]},"reference":[{"key":"e_1_2_2_1_2","unstructured":"\u00a0"},{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1039\/b801747f"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200806121"},{"key":"e_1_2_2_3_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200806121"},{"key":"e_1_2_2_4_2","unstructured":"\u00a0"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2np20019h"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi400988t"},{"key":"e_1_2_2_7_2","unstructured":"\u00a0"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R110.144618"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16076092"},{"key":"e_1_2_2_10_2","unstructured":"\u00a0"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200500430"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200600285"},{"key":"e_1_2_2_13_2","unstructured":"\u00a0"},{"key":"e_1_2_2_14_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.49.1196"},{"key":"e_1_2_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2007.18"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja105649g"},{"key":"e_1_2_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2004.08.018"},{"key":"e_1_2_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2012.07.024"},{"key":"e_1_2_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806305105"},{"key":"e_1_2_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201205041"},{"key":"e_1_2_2_20_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201205041"},{"key":"e_1_2_2_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja078081n"},{"key":"e_1_2_2_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/np300913h"},{"key":"e_1_2_2_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201300068"},{"key":"e_1_2_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1213892110"},{"key":"e_1_2_2_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03523.x"},{"key":"e_1_2_2_26_2","unstructured":"\u00a0"},{"key":"e_1_2_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2005.01.009"},{"key":"e_1_2_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201100474"},{"key":"e_1_2_2_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr300169a"},{"key":"e_1_2_2_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205484200"},{"key":"e_1_2_2_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.7.4965"},{"key":"e_1_2_2_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0640187"},{"key":"e_1_2_2_33_2","unstructured":"\u00a0"},{"key":"e_1_2_2_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol301785x"},{"key":"e_1_2_2_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb300416w"},{"key":"e_1_2_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-2797(02)00164-3"},{"key":"e_1_2_2_37_2","unstructured":"\u00a0"},{"key":"e_1_2_2_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200300581"},{"key":"e_1_2_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2007.07.009"},{"key":"e_1_2_2_40_2","doi-asserted-by":"publisher","DOI":"10.1039\/B801658P"}],"container-title":["Angewandte 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