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A new universal method has been devised to prepare these compounds by using a set of engineered marine\u2010derived enzymes and substrates obtained from a family of ribosomally produced and post\u2010translationally modified peptides called the cyanobactins. The substrate precursor peptide is engineered to have a non\u2010native protease cleavage site that can be rapidly cleaved. The other enzymes used are heterocyclases that convert Cys or Cys\/Ser\/Thr into their corresponding azolines. A macrocycle is formed using a macrocyclase enzyme, followed by oxidation of the azolines to azoles with a specific oxidase. The work is exemplified by the production of 17 macrocycles containing 6\u20139 residues representing 11 out of the 20 canonical amino acids.<\/jats:p>","DOI":"10.1002\/anie.201408082","type":"journal-article","created":{"date-parts":[[2014,10,21]],"date-time":"2014-10-21T09:47:21Z","timestamp":1413884841000},"page":"14171-14174","source":"Crossref","is-referenced-by-count":60,"title":["An Efficient Method for the In Vitro Production of Azol(in)e\u2010Based Cyclic Peptides"],"prefix":"10.1002","volume":"53","author":[{"given":"Wael E.","family":"Houssen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew F.","family":"Bent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R.","family":"McEwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Pieiller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jioji","family":"Tabudravu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesko","family":"Koehnke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Mann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary I.","family":"Adaba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usama W.","family":"Hawas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanting","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Schwarz\u2010Linek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret C. 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