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To understand and investigate the role of individual components in such signaling networks, the availability of specific inhibitors is of paramount importance. We report the generation and validation of a novel variant of an RNA aptamer that selectively inhibits the mitogen\u2010activated kinase pathway in neurons. We demonstrate that the aptamer retains function under intracellular conditions and that application of the aptamer through the patch\u2010clamp pipette efficiently inhibits mitogen\u2010activated kinase\u2010dependent synaptic plasticity. This approach introduces synthetic aptamers as generic tools, readily applicable to inhibit different components of intraneuronal signaling networks with utmost specificity.<\/jats:p>","DOI":"10.1002\/anie.201409597","type":"journal-article","created":{"date-parts":[[2015,3,5]],"date-time":"2015-03-05T20:27:13Z","timestamp":1425587233000},"page":"5369-5373","source":"Crossref","is-referenced-by-count":9,"title":["Selective Aptamer\u2010Based Control of Intraneuronal Signaling"],"prefix":"10.1002","volume":"54","author":[{"given":"Sabine","family":"Lennarz","sequence":"first","affiliation":[{"name":"Life and Medical Sciences Institute University of Bonn  Gerhard-Domagk-Str. 1 Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Therese Christine","family":"Alich","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Epileptology and Cognition Research Department of Epileptology  Sigmund-Freud Str. 25 Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Kelly","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Epileptology and Cognition Research Department of Epileptology  Sigmund-Freud Str. 25 Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Blind","sequence":"additional","affiliation":[{"name":"Life and Medical Sciences Institute University of Bonn  Gerhard-Domagk-Str. 1 Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinz","family":"Beck","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Epileptology and Cognition Research Department of Epileptology  Sigmund-Freud Str. 25 Bonn Germany"},{"name":"Deutsches Zentrum f\u00fcr Neurodegenerative Erkrankungen in der Helmholtz Gemeinschaft"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnter","family":"Mayer","sequence":"additional","affiliation":[{"name":"Life and Medical Sciences Institute University of Bonn  Gerhard-Domagk-Str. 1 Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,5]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81688-8"},{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.228"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-013-8489-4"},{"key":"e_1_2_2_4_1","unstructured":"\u00a0"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi982515x"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.091100698"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402901101"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2012.158"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-09-0214"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-1128"},{"key":"e_1_2_2_11_1","unstructured":"\u00a0"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi002376v"},{"key":"e_1_2_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2701"},{"key":"e_1_2_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2970"},{"key":"e_1_2_2_15_1","unstructured":"\u00a0"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1210\/er.22.2.153"},{"key":"e_1_2_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2012.04.005"},{"key":"e_1_2_2_18_1","unstructured":"\u00a0"},{"key":"e_1_2_2_19_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.158816"},{"key":"e_1_2_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2004.04.001"},{"key":"e_1_2_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1346"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1097065"},{"key":"e_1_2_2_23_1","doi-asserted-by":"crossref","first-page":"613","DOI":"10.3233\/JAD-2009-1167","volume":"18","author":"Klafki H.-W.","year":"2009","journal-title":"J. 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