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A luminescence resonance energy transfer (LRET) probe for biothiols was constructed by decorating UCPs with dithiol\u2010stabilized Ag\u2005NCs. Owing to the unique properties of ultrasmall NCs, properties which bridge the gap between those of small molecules and those of nanoparticles, the use of approximately 1.9\u2005nm Ag\u2005NCs as energy acceptors endows the probe with high energy\u2010transfer efficiency, good biocompatibility, and flexibility. The UCP\u2013Ag\u2005NC nanoprobe enables rapid and robust target assay in solutions. It was also uploaded into living cells and used to detect intracellular biothiol levels with high discrimination. Moreover, the probe shows transportability in\u2005vivo and can be used for tissue imaging. The facile growth of few\u2010atom metal NCs on diverse templates may enable the development of various nanoprobes combining UCPs and metal NCs.<\/jats:p>","DOI":"10.1002\/anie.201500008","type":"journal-article","created":{"date-parts":[[2015,3,5]],"date-time":"2015-03-05T20:27:23Z","timestamp":1425587243000},"page":"5323-5327","source":"Crossref","is-referenced-by-count":88,"title":["Construction of an Upconversion Nanoprobe with Few\u2010Atom Silver Nanoclusters as the Energy Acceptor"],"prefix":"10.1002","volume":"54","author":[{"given":"Yan","family":"Xiao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyu","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengjun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,5]]},"reference":[{"key":"e_1_2_2_1_2","unstructured":"\u00a0"},{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja2102604"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201005159"},{"key":"e_1_2_2_3_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201005159"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201004902"},{"key":"e_1_2_2_4_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201004902"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja5042989"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201109156"},{"key":"e_1_2_2_6_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201109156"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac5009207"},{"key":"e_1_2_2_8_2","unstructured":"\u00a0"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201102981"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac5016694"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja205907y"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/nn202620u"},{"key":"e_1_2_2_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/am501249p"},{"key":"e_1_2_2_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201403408"},{"key":"e_1_2_2_14_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201403408"},{"key":"e_1_2_2_15_2","unstructured":"\u00a0"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0644024"},{"key":"e_1_2_2_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja076151k"},{"key":"e_1_2_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200705861"},{"key":"e_1_2_2_18_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200705861"},{"key":"e_1_2_2_19_2","unstructured":"\u00a0"},{"key":"e_1_2_2_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac301534w"},{"key":"e_1_2_2_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac202627b"},{"key":"e_1_2_2_22_2","unstructured":"\u00a0"},{"key":"e_1_2_2_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200501907"},{"key":"e_1_2_2_23_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200501907"},{"key":"e_1_2_2_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac901808q"},{"key":"e_1_2_2_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja2100774"},{"key":"e_1_2_2_26_2","unstructured":"\u00a0"},{"key":"e_1_2_2_27_2","doi-asserted-by":"publisher","DOI":"10.1039\/c1nr00006c"},{"key":"e_1_2_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201200053"},{"key":"e_1_2_2_29_2","doi-asserted-by":"publisher","DOI":"10.1039\/C1CS15226B"},{"key":"e_1_2_2_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja403485r"},{"key":"e_1_2_2_31_2","unstructured":"\u00a0"},{"key":"e_1_2_2_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja4035939"},{"key":"e_1_2_2_33_2","first-page":"2303","volume":"135","author":"Zhang L.","year":"2013","journal-title":"J. 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