{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:02:42Z","timestamp":1792782162816,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"35","license":[{"start":{"date-parts":[[2015,6,26]],"date-time":"2015-06-26T00:00:00Z","timestamp":1435276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001655","name":"DAAD","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"crossref"}]},{"name":"TMBWK","award":["43-5572-321-12040-12"],"award-info":[{"award-number":["43-5572-321-12040-12"]}]},{"name":"DFG"},{"DOI":"10.13039\/501100000780","name":"European commission","doi-asserted-by":"crossref","award":["311477"],"award-info":[{"award-number":["311477"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2015,8,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The largest continuous bacterial nonribosomal peptide synthetase discovered so far is described. It consists of 15\u2005consecutive modules arising from an uninterrupted, fully functional gene in the entomopathogenic bacterium\n                    <jats:italic>Photorhabdus luminescens<\/jats:italic>\n                    . The identification of its cryptic biosynthesis product was achieved by using a combination of genome analysis, promoter exchange, isotopic labeling experiments, and total synthesis of a focused collection of peptide candidates. Although it belongs to the growing class of\n                    <jats:sc>D<\/jats:sc>\n                    \u2010\/\u2009\n                    <jats:sc>L<\/jats:sc>\n                    \u2010peptide natural products, the encoded metabolite kolossin\u2005A was found to be largely devoid of antibiotic activity and is likely involved in interspecies communication. A stereoisomer of this peculiar natural product displayed high activity against\n                    <jats:italic>Trypanosoma brucei rhodesiense<\/jats:italic>\n                    , a recalcitrant parasite that causes the deadly disease African sleeping sickness.\n                  <\/jats:p>","DOI":"10.1002\/anie.201502835","type":"journal-article","created":{"date-parts":[[2015,6,27]],"date-time":"2015-06-27T04:57:12Z","timestamp":1435381032000},"page":"10352-10355","source":"Crossref","is-referenced-by-count":74,"title":["Structure Elucidation and Activity of Kolossin\u2005A, the\n                    <scp>D<\/scp>\n                    \u2010\/\n                    <scp>L<\/scp>\n                    \u2010Pentadecapeptide Product of a Giant Nonribosomal Peptide Synthetase"],"prefix":"10.1002","volume":"54","author":[{"given":"Helge B.","family":"Bode","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander O.","family":"Brachmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirtikumar B.","family":"Jadhav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Seyfarth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Dauth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian W.","family":"Fuchs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Kaiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick R.","family":"Waterfield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Sack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan H.","family":"Heinemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans\u2010Dieter","family":"Arndt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,26]]},"reference":[{"key":"e_1_2_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2011.10.002"},{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja1087369"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090110-102805"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1319584111"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.684"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb900187p"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac300372p"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201300602"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2717"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt449"},{"key":"e_1_2_2_11_2","first-page":"123","volume":"135","author":"Brachmann A. 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