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In this work we exploit femtosecond to microsecond transient IR spectroscopy to record, in D<jats:sub>2<\/jats:sub>O, the complete proton transfer photocycle of avGFP, and two mutants (T203V and S205V) which modify the structure of the proton wire. Striking differences and similarities are observed among the three mutants yielding novel information on proton transfer mechanism, rates, isotope effects, H\u2010bond strength and proton wire stability. These data provide a detailed picture of the dynamics of long\u2010range proton transfer in a protein against which calculations may be compared.<\/jats:p>","DOI":"10.1002\/anie.201503672","type":"journal-article","created":{"date-parts":[[2015,6,18]],"date-time":"2015-06-18T20:28:57Z","timestamp":1434659337000},"page":"9303-9307","source":"Crossref","is-referenced-by-count":32,"title":["Complete Proton Transfer Cycle in GFP and Its T203V and S205V Mutants"],"prefix":"10.1002","volume":"54","author":[{"given":"Sergey 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