{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T20:36:27Z","timestamp":1776371787315,"version":"3.51.2"},"reference-count":53,"publisher":"Wiley","issue":"46","license":[{"start":{"date-parts":[[2015,9,17]],"date-time":"2015-09-17T00:00:00Z","timestamp":1442448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funded Access"},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"crossref","award":["BB\/J007250\/1"],"award-info":[{"award-number":["BB\/J007250\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000735","name":"University of Cambridge","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000735","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Todd-Raphael Fund","award":["2010\/15971-3"],"award-info":[{"award-number":["2010\/15971-3"]}]},{"name":"Todd-Raphael Fund","award":["2011\/17510-6"],"award-info":[{"award-number":["2011\/17510-6"]}]},{"name":"S\u00e3o Paolo Research Foundation (FAPESP) of Brazil"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2015,11,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tetrahydropyran rings are a common feature of complex polyketide natural products, but much remains to be learned about the enzymology of their formation. The enzyme SalBIII from the salinomycin biosynthetic pathway resembles other polyether epoxide hydrolases\/cyclases of the MonB family, but SalBIII plays no role in the conventional cascade of ring opening\/closing. Mutation in the <jats:italic>salBIII<\/jats:italic> gene gave a metabolite in which ring\u2005A is not formed. Using this metabolite in\u2005vitro as a substrate analogue, SalBIII has been shown to form pyran ring\u2005A. We have determined the X\u2010ray crystal structure of SalBIII, and structure\u2010guided mutagenesis of putative active\u2010site residues has identified Asp38 and Asp104 as an essential catalytic dyad. The demonstrated pyran synthase activity of SalBIII further extends the impressive catalytic versatility of \u03b1+\u03b2 barrel fold proteins.<\/jats:p>","DOI":"10.1002\/anie.201507090","type":"journal-article","created":{"date-parts":[[2015,9,17]],"date-time":"2015-09-17T11:36:00Z","timestamp":1442489760000},"page":"13622-13625","source":"Crossref","is-referenced-by-count":43,"title":["Enzymology of Pyran Ring\u2005A Formation in Salinomycin Biosynthesis"],"prefix":"10.1002","volume":"54","author":[{"given":"Hanna","family":"Luhavaya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcio V. B.","family":"Dias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon R.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana G.","family":"de\u2005Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter F.","family":"Leadlay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,17]]},"reference":[{"key":"e_1_2_3_1_2","unstructured":"\u00a0"},{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.06.034"},{"key":"e_1_2_3_3_2","doi-asserted-by":"publisher","DOI":"10.2174\/15672050113109990199"},{"key":"e_1_2_3_4_2","unstructured":"\u00a0"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.1039\/a909079g"},{"key":"e_1_2_3_6_2","doi-asserted-by":"publisher","DOI":"10.1039\/b100191o"},{"key":"e_1_2_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(09)04606-0"},{"key":"e_1_2_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200806121"},{"key":"e_1_2_3_8_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200806121"},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr0503097"},{"key":"e_1_2_3_10_2","unstructured":"\u00a0"},{"key":"e_1_2_3_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-27055-8_3"},{"key":"e_1_2_3_12_2","doi-asserted-by":"publisher","DOI":"10.1039\/b805115c"},{"key":"e_1_2_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.12.018"},{"key":"e_1_2_3_14_2","unstructured":"\u00a0"},{"key":"e_1_2_3_15_2","doi-asserted-by":"publisher","DOI":"10.1039\/b816430b"},{"key":"e_1_2_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1379"},{"key":"e_1_2_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2009.05.021"},{"key":"e_1_2_3_18_2","unstructured":"\u00a0"},{"key":"e_1_2_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1287"},{"key":"e_1_2_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.05.005"},{"key":"e_1_2_3_21_2","unstructured":"\u00a0"},{"key":"e_1_2_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jim.7000204"},{"key":"e_1_2_3_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03571.x"},{"key":"e_1_2_3_24_2","unstructured":"\u00a0"},{"key":"e_1_2_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2006.01.013"},{"key":"e_1_2_3_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200800585"},{"key":"e_1_2_3_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja301386g"},{"key":"e_1_2_3_28_2","unstructured":"\u00a0"},{"key":"e_1_2_3_29_2","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1128\/AAC.43.7.1662","volume":"43","author":"Woo A. J.","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_2_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1809-1817.2000"},{"key":"e_1_2_3_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405976101"},{"key":"e_1_2_3_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/np060361d"},{"key":"e_1_2_3_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201000313"},{"key":"e_1_2_3_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201307406"},{"key":"e_1_2_3_34_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201307406"},{"key":"e_1_2_3_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201407979"},{"key":"e_1_2_3_35_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201407979"},{"key":"e_1_2_3_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200800822"},{"key":"e_1_2_3_37_2","unstructured":"\u00a0"},{"key":"e_1_2_3_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201100590"},{"key":"e_1_2_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06701-11"},{"key":"e_1_2_3_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201402300"},{"key":"e_1_2_3_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201503561"},{"key":"e_1_2_3_41_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201503561"},{"key":"e_1_2_3_42_2","unstructured":"\u00a0"},{"key":"e_1_2_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(94)00095-6"},{"key":"e_1_2_3_44_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi971546"},{"key":"e_1_2_3_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg275"},{"key":"e_1_2_3_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600201"},{"key":"e_1_2_3_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10865"},{"key":"e_1_2_3_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb4006485"},{"key":"e_1_2_3_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00077a062"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fanie.201507090","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.201507090","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:46:48Z","timestamp":1697503608000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.201507090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,17]]},"references-count":53,"journal-issue":{"issue":"46","published-print":{"date-parts":[[2015,11,9]]}},"alternative-id":["10.1002\/anie.201507090"],"URL":"https:\/\/doi.org\/10.1002\/anie.201507090","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,17]]}}}