{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:31:35Z","timestamp":1790839895463,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"50","license":[{"start":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T00:00:00Z","timestamp":1445558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"JST"},{"DOI":"10.13039\/501100003382","name":"CREST","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003382","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Japan Advanced Plant Science Network"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2015,12,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The development of stimulated emission depletion (STED) microscopy represented a major breakthrough in cellular and molecular biology. However, the intense laser beams required for both excitation and STED usually provoke rapid photobleaching of fluorescent molecular probes, which significantly limits the performance and practical utility of STED microscopy. We herein developed a photoresistant fluorescent dye C\u2010Naphox as a practical tool for STED imaging. With excitation using either a <jats:italic>\u03bb<\/jats:italic>=405\u2005or 488\u2005nm laser in protic solvents, C\u2010Naphox exhibited an intense red\/orange fluorescence (quantum yield <jats:italic>\u03a6<\/jats:italic><jats:sub>F<\/jats:sub>&gt;0.7) with a large Stokes shift (circa 5900\u2005cm<jats:sup>\u22121<\/jats:sup>). Even after irradiation with a Xe\u2005lamp (300\u2005W, <jats:italic>\u03bb<\/jats:italic><jats:sub>ex<\/jats:sub>=460\u2005nm, full width at half maximum (FWHM)=11\u2005nm) for 12\u2005hours, 99.5\u2009% of C\u2010Naphox remained intact. The high photoresistance of C\u2010Naphox allowed repeated STED imaging of HeLa cells. Even after recording 50 STED\u2005images, 83\u2009% of the initial fluorescence intensity persisted.<\/jats:p>","DOI":"10.1002\/anie.201507939","type":"journal-article","created":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T11:27:57Z","timestamp":1445599677000},"page":"15213-15217","source":"Crossref","is-referenced-by-count":136,"title":["A Phosphole Oxide Based Fluorescent Dye with Exceptional Resistance to Photobleaching: A Practical Tool for Continuous Imaging in STED Microscopy"],"prefix":"10.1002","volume":"54","author":[{"given":"Chenguang","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiko","family":"Fukazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayasu","family":"Taki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikatsu","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Higashiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigehiro","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,23]]},"reference":[{"key":"e_1_2_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2014.03.021"},{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201002018"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2531"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.201100986"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12243"},{"key":"e_1_2_4_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201403349"},{"key":"e_1_2_4_6_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201403349"},{"key":"e_1_2_4_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0807705105"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1546"},{"key":"e_1_2_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2972"},{"key":"e_1_2_4_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.15.8206"},{"key":"e_1_2_4_11_2","doi-asserted-by":"publisher","DOI":"10.1364\/AO.42.005123"},{"key":"e_1_2_4_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201500229"},{"key":"e_1_2_4_12_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201500229"},{"key":"e_1_2_4_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.joc.5b00541"},{"key":"e_1_2_4_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/asia.200900303"},{"key":"e_1_2_4_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201302037"},{"key":"e_1_2_4_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201201168"},{"key":"e_1_2_4_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12154-013-0096-3"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fanie.201507939","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.201507939","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T18:35:55Z","timestamp":1697481355000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.201507939"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,23]]},"references-count":19,"journal-issue":{"issue":"50","published-print":{"date-parts":[[2015,12,7]]}},"alternative-id":["10.1002\/anie.201507939"],"URL":"https:\/\/doi.org\/10.1002\/anie.201507939","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,23]]}}}