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Herein, we report a single biosensor for electrochemical ratiometric detection of Cu<jats:sup>2+<\/jats:sup> and CySH in live rat brains with AD. <jats:italic>N<\/jats:italic>,<jats:italic>N<\/jats:italic>\u2010di\u2010(2\u2010picoly)ethylenediamine (DPEA) is first synthesized for specific recognition of Cu<jats:sup>2+<\/jats:sup> to form a DPEA\u2013Cu<jats:sup>2+<\/jats:sup> complex. This complex shows high selectivity for CySH owing to the release of Cu<jats:sup>2+<\/jats:sup> from the complex through CySH binding to Cu<jats:sup>2+<\/jats:sup> center. In parallel, 5\u2032\u2010MB\u2010GGCGCGATTTTTTTTTTTTT\u2010SH\u20103\u2032 (HS\u2010DNA\u2010MB, MB=Methylene Blue) is designed as an inner\u2010reference for providing a built\u2010in correction to improve the accuracy. As a result, combined with the amplified effect of Au nanoleaves, our single ratiometric biosensor can be successfully applied in real\u2010time detection of Cu<jats:sup>2+<\/jats:sup> and CySH in the live rat brains with AD. To our knowledge, this is the first report on the accurate concentrations of Cu<jats:sup>2+<\/jats:sup> and CySH in live rat brains with AD.<\/jats:p>","DOI":"10.1002\/anie.201508635","type":"journal-article","created":{"date-parts":[[2015,10,21]],"date-time":"2015-10-21T11:34:39Z","timestamp":1445427279000},"page":"14053-14056","source":"Crossref","is-referenced-by-count":148,"title":["A Single Biosensor for Evaluating the Levels of Copper Ion and <scp>L<\/scp>\u2010Cysteine in a Live Rat Brain with Alzheimer's Disease"],"prefix":"10.1002","volume":"54","author":[{"given":"Yongping","family":"Luo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,21]]},"reference":[{"key":"e_1_2_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-1693(03)00471-7"},{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(03)00067-5"},{"key":"e_1_2_3_3_2","unstructured":"\u00a0"},{"key":"e_1_2_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201410389"},{"key":"e_1_2_3_4_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201410389"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201408810"},{"key":"e_1_2_3_5_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201408810"},{"key":"e_1_2_3_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0740270.x"},{"key":"e_1_2_3_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ic301865n"},{"key":"e_1_2_3_8_2","unstructured":"\u00a0"},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2006.06.001"},{"key":"e_1_2_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2006.03.005"},{"key":"e_1_2_3_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-006-0454-x"},{"key":"e_1_2_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.02.024"},{"key":"e_1_2_3_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200802317"},{"key":"e_1_2_3_13_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200802317"},{"key":"e_1_2_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(02)00649-5"},{"key":"e_1_2_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201109089"},{"key":"e_1_2_3_15_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201109089"},{"key":"e_1_2_3_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja907778b"},{"key":"e_1_2_3_17_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2cc31000g"},{"key":"e_1_2_3_18_2","unstructured":"\u00a0"},{"key":"e_1_2_3_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac303113n"},{"key":"e_1_2_3_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac504448m"},{"key":"e_1_2_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201302958"},{"key":"e_1_2_3_21_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201302958"},{"key":"e_1_2_3_22_2","unstructured":"\u00a0"},{"key":"e_1_2_3_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac201761c"},{"key":"e_1_2_3_24_2","volume-title":"Laboratory Techniques in Electroanalytical Chemistry","author":"McCreery R. 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