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We have proposed that desertomycin\u2005A and similar compounds (marginolactones) are formed by polyketide synthases primed not with \u03b3\u2010aminobutanoyl\u2010CoA but with 4\u2010guanidinylbutanoyl\u2010CoA, to avoid facile cyclization of the starter unit. This hypothesis requires that there be a final\u2010stage de\u2010amidination of the corresponding guanidino\u2010substituted natural product, but no enzyme for such a process has been described. We have now identified candidate amidinohydrolase genes within the desertomycin and primycin clusters. Deletion of the putative desertomycin amidinohydrolase gene dstH in Streptomyces macronensis led to the accumulation of desertomycin\u2005B, the guanidino form of the antibiotic. Also, purified DstH efficiently catalyzed the in\u2005vitro conversion of desertomycin\u2005B into the A form. Hence this amidinohydrolase furnishes the missing link in this proposed naturally evolved example of protective\u2010group chemistry.<\/jats:p>","DOI":"10.1002\/anie.201509300","type":"journal-article","created":{"date-parts":[[2015,12,2]],"date-time":"2015-12-02T13:47:15Z","timestamp":1449064035000},"page":"1118-1123","source":"Crossref","is-referenced-by-count":31,"title":["An Amidinohydrolase Provides the Missing Link in the Biosynthesis of Amino Marginolactone Antibiotics"],"prefix":"10.1002","volume":"55","author":[{"given":"Hui","family":"Hong","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Cambridge  80 Tennis Court Road Cambridge CB2 1GA UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markiyan","family":"Samborskyy","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Cambridge  80 Tennis Court Road Cambridge CB2 1GA UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick","family":"Lindner","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Cambridge  80 Tennis Court Road Cambridge CB2 1GA UK"},{"name":"Institut f\u00fcr Organische Chemie Leibniz Universit\u00e4t Hannover  Schneiderberg 1 B 30167 Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter F.","family":"Leadlay","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Cambridge  80 Tennis Court Road Cambridge CB2 1GA UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,2]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200806121"},{"key":"e_1_2_2_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200806121"},{"key":"e_1_2_2_2_1","unstructured":"\u00a0"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1039\/a909079g"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1039\/b100191o"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(09)04606-0"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr0503097"},{"key":"e_1_2_2_7_1","unstructured":"\u00a0"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1039\/b514050c"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2007.7"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200800389"},{"key":"e_1_2_2_11_1","unstructured":"\u00a0"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1287"},{"key":"e_1_2_2_13_2","first-page":"748","volume":"8","author":"Wilkinson B.","year":"2005","journal-title":"Curr. 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