{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:11:51Z","timestamp":1791310311875,"version":"4.3.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,12,3]],"date-time":"2015-12-03T00:00:00Z","timestamp":1449100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"crossref","award":["17242"],"award-info":[{"award-number":["17242"]}],"id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"crossref"}]},{"name":"CRUK-EPSRC","award":["16465"],"award-info":[{"award-number":["16465"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2016,1,22]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glycosylation is a ubiquitous post\u2010translational modification, present in over 50\u2009% of the proteins in the human genome,<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#anie201509858-bib-0001\"\/> with important roles in cell\u2013cell communication and migration. Interest in glycome profiling has increased with the realization that glycans can be used as biomarkers of many diseases,<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#anie201509858-bib-0002\"\/> including cancer.<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#anie201509858-bib-0003\"\/> We report here the first tomographic imaging of glycosylated tissues in live mice by using metabolic labeling and a gadolinium\u2010based bioorthogonal MRI probe. Significant <jats:italic>N<\/jats:italic>\u2010azidoacetylgalactosamine dependent <jats:italic>T<\/jats:italic><jats:sub>1<\/jats:sub>\u2005contrast was observed in\u2005vivo two hours after probe administration. Tumor, kidney, and liver showed significant contrast, and several other tissues, including the pancreas, spleen, heart, and intestines, showed a very high contrast (&gt;10\u2010fold). This approach has the potential to enable the rapid and non\u2010invasive magnetic resonance imaging of glycosylated tissues in\u2005vivo in preclinical models of disease.<\/jats:p>","DOI":"10.1002\/anie.201509858","type":"journal-article","created":{"date-parts":[[2015,12,3]],"date-time":"2015-12-03T14:00:53Z","timestamp":1449151253000},"page":"1286-1290","source":"Crossref","is-referenced-by-count":32,"title":["Imaging Glycosylation In Vivo by Metabolic Labeling and Magnetic Resonance Imaging"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2740-5166","authenticated-orcid":false,"given":"Andr\u00e9 A.","family":"Neves","sequence":"first","affiliation":[{"name":"Cancer Research UK Cambridge Institute Li Ka Shing Centre  Cambridge CB2 0RE UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y\u00e9l\u00e9na A.","family":"Wainman","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Institute Li Ka Shing Centre  Cambridge CB2 0RE UK"},{"name":"Department of Chemistry University of Cambridge  Cambridge CB2 1EW UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Wright","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Institute Li Ka Shing Centre  Cambridge CB2 0RE UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikko I.","family":"Kettunen","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Institute Li Ka Shing Centre  Cambridge CB2 0RE UK"},{"name":"A. 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Virtanen Institute for Molecular Sciences University of Eastern Finland  Neulaniementie 2 70211 Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago B.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Institute Li Ka Shing Centre  Cambridge CB2 0RE UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"McGuire","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Institute Li Ka Shing Centre  Cambridge CB2 0RE UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De\u2010En","family":"Hu","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Institute Li Ka Shing Centre  Cambridge CB2 0RE UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flaviu","family":"Bulat","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Cambridge  Cambridge CB2 1EW 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