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Recently we identified several \u03b1\u2010conotoxins that also modulate voltage\u2010gated calcium channels by acting as G protein\u2010coupled GABA<jats:sub>B<\/jats:sub> receptor (GABA<jats:sub>B<\/jats:sub>R) agonists. These \u03b1\u2010conotoxins are promising drug leads for the treatment of chronic pain. To elucidate the diversity of \u03b1\u2010conotoxins that act through this mechanism, we synthesized and characterized a set of peptides with homology to \u03b1\u2010conotoxins known to inhibit high voltage\u2010activated calcium channels via GABA<jats:sub>B<\/jats:sub>R activation. Remarkably, all disulfide isomers of the active \u03b1\u2010conotoxins Pu1.2 and Pn1.2, and the previously studied Vc1.1 showed similar levels of biological activity. 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