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Herein, we propose a MALDI\u2010MS patterning strategy for the convenient visual presentation of multiple enzyme activities with an easy\u2010to\u2010prepare chip. The array\u2010based caspase\u2010activity patterned chip (Casp\u2010PC) is fabricated by hydrophobically assembling different phospholipid\u2010tagged peptide substrates on a modified ITO slide. The advantages of amphipathic phospholipids lead to high\u2010quality mass spectra for imaging analysis. Upon the respective cleavage of these substrates by different caspases, such as caspase\u20101, \u20102, \u20103, and \u20108, to produce a mass shift, the enzyme activities can be directly evaluated by MALDI\u2010MS patterning by <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic>\u2010dependent imaging of the cleavage products. The ability to identify drug\u2010sensitive\/resistant cancer cells and assess the curative effects of anticancer drugs is demonstrated, indicating the applicability of the method and the designed chip.<\/jats:p>","DOI":"10.1002\/anie.201601096","type":"journal-article","created":{"date-parts":[[2016,4,22]],"date-time":"2016-04-22T01:26:10Z","timestamp":1461288370000},"page":"6667-6670","source":"Crossref","is-referenced-by-count":43,"title":["MALDI\u2010MS Patterning of Caspase Activities and Its Application in the Assessment of Drug Resistance"],"prefix":"10.1002","volume":"55","author":[{"given":"Junjie","family":"Hu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering Nanjing University  Nanjing 210023 P.R. 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