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Current methods to detect CTCs are based on immunostaining or discrimination of physical properties. Herein, a label\u2010free method is presented exploiting the abnormal metabolic behavior of cancer cells. A single\u2010cell analysis technique is used to measure the secretion of acid from individual living tumor cells compartmentalized in microfluidically prepared, monodisperse, picoliter (pL) droplets. As few as 10 tumor cells can be detected in a background of 200\u2009000 white blood cells and proof\u2010of\u2010concept data is shown on the detection of CTCs in the blood of metastatic patients.<\/jats:p>","DOI":"10.1002\/anie.201602328","type":"journal-article","created":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T12:12:31Z","timestamp":1464783151000},"page":"8581-8584","source":"Crossref","is-referenced-by-count":112,"title":["A Method for Detecting Circulating Tumor Cells Based on the Measurement of Single\u2010Cell Metabolism in Droplet\u2010Based Microfluidics"],"prefix":"10.1002","volume":"55","author":[{"given":"Fabio","family":"Del\u2005Ben","sequence":"first","affiliation":[{"name":"Graduate School of Nanotechnology University of Trieste  Via Valerio 2 Trieste Italy"},{"name":"Dept. of Clinical Pathology CRO Aviano  Via F. 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