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Detergent solubilization may significantly alter or even abolish such hetero\u2010oligomeric interactions, which makes observing them at high resolution in their native environment technically challenging. Dipolar electron paramagnetic resonance (EPR) techniques such as pulsed electro\u2013electron double resonance (PELDOR) can provide very precise distances within biomolecules. To concurrently determine the inter\u2010subunit interaction and the intra\u2010subunit conformational changes in hetero\u2010oligomeric complexes, a combination of different spin labels is required. Orthogonal spin labeling using a triarylmethyl (TAM) label in combination with a nitroxide label is used to detect protein\u2013ligand interactions in native lipid bilayers. This approach provides a higher sensitivity and total selectivity and will greatly facilitate the investigation of multimeric transmembrane complexes employing different spin labels in the native lipid environment.<\/jats:p>","DOI":"10.1002\/anie.201606335","type":"journal-article","created":{"date-parts":[[2016,8,11]],"date-time":"2016-08-11T07:05:52Z","timestamp":1470899152000},"page":"11538-11542","source":"Crossref","is-referenced-by-count":91,"title":["Selective High\u2010Resolution Detection of Membrane Protein\u2013Ligand Interaction in Native Membranes Using Trityl\u2013Nitroxide PELDOR"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4968-889X","authenticated-orcid":false,"given":"Benesh","family":"Joseph","sequence":"first","affiliation":[{"name":"Institut f\u00fcr physikalische und theoretische Chemie und Biomolekulares Magnetresonanz Zentrum Universit\u00e4t Frankfurt  Max-von-Laue-Strasse 7 60438 Frankfurt am Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4975-9993","authenticated-orcid":false,"given":"Victor M.","family":"Tormyshev","sequence":"additional","affiliation":[{"name":"N. 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