{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:30:10Z","timestamp":1786977010980,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2017,3,15]],"date-time":"2017-03-15T00:00:00Z","timestamp":1489536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 104-2113-M-259-006"],"award-info":[{"award-number":["MOST 104-2113-M-259-006"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 104-0210-01-09-02"],"award-info":[{"award-number":["MOST 104-0210-01-09-02"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 104-2628-M-001-001"],"award-info":[{"award-number":["MOST 104-2628-M-001-001"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 105-0210-01-13-01"],"award-info":[{"award-number":["MOST 105-0210-01-13-01"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 105-2745-M-001-003-ASP"],"award-info":[{"award-number":["MOST 105-2745-M-001-003-ASP"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2017,4,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Heparin\u2010binding hemagglutinin (HBHA) is a 199 amino acid virulence factor at the envelope of\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    that contributes to latent tuberculosis. The binding of HBHA to respiratory epithelial cells, which leads to extrapulmonary dissemination of the pathogen, is mediated by cell\u2010surface heparan sulfate (HS). We report the structural characterization of the HBHA\/HS complex by NMR spectroscopy. To develop a model for the molecular recognition, the first chemically synthesized uniformly\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010 and\n                    <jats:sup>15<\/jats:sup>\n                    N\u2010labeled HS octasaccharide and a uniformly\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010 and\n                    <jats:sup>15<\/jats:sup>\n                    N\u2010labeled form of HBHA were prepared. Residues 180\u2013195 at the C\u2010terminal region of HBHA show large chemical shift perturbation upon association with the octasaccharide. Molecular dynamics simulations conforming to the multidimensional NMR data revealed key electrostatic and even hydrophobic interactions between the binding partners that may aid in the development of agents targeting the binding event.\n                  <\/jats:p>","DOI":"10.1002\/anie.201612518","type":"journal-article","created":{"date-parts":[[2017,3,15]],"date-time":"2017-03-15T04:32:44Z","timestamp":1489552364000},"page":"4192-4196","source":"Crossref","is-referenced-by-count":30,"title":["Structure of the Complex between a Heparan Sulfate Octasaccharide and Mycobacterial Heparin\u2010Binding Hemagglutinin"],"prefix":"10.1002","volume":"56","author":[{"given":"Teng\u2010Yi","family":"Huang","sequence":"first","affiliation":[{"name":"Genomics Research Center Academia Sinica  No. 128, Section 2, Academia Road Taipei 115 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deli","family":"Irene","sequence":"additional","affiliation":[{"name":"Department of Chemistry National Dong Hwa University  No. 1, Section 2, Da Hsueh Road, Shoufeng Hualien 974 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Medel Manuel L.","family":"Zulueta","sequence":"additional","affiliation":[{"name":"Genomics Research Center Academia Sinica  No. 128, Section 2, Academia Road Taipei 115 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu\u2010Jui","family":"Tai","sequence":"additional","affiliation":[{"name":"Department of Chemistry National Dong Hwa University  No. 1, Section 2, Da Hsueh Road, Shoufeng Hualien 974 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih\u2010Han","family":"Lain","sequence":"additional","affiliation":[{"name":"Department of Chemistry National Dong Hwa University  No. 1, Section 2, Da Hsueh Road, Shoufeng Hualien 974 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Po","family":"Cheng","sequence":"additional","affiliation":[{"name":"Genomics Research Center Academia Sinica  No. 128, Section 2, Academia Road Taipei 115 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping\u2010Xi","family":"Tsai","sequence":"additional","affiliation":[{"name":"Genomics Research Center Academia Sinica  No. 128, Section 2, Academia Road Taipei 115 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu\u2010Yi","family":"Lin","sequence":"additional","affiliation":[{"name":"Genomics Research Center Academia Sinica  No. 128, Section 2, Academia Road Taipei 115 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Geng","family":"Chen","sequence":"additional","affiliation":[{"name":"Genomics Research Center Academia Sinica  No. 128, Section 2, Academia Road Taipei 115 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiao\u2010Chu","family":"Ku","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology Academia Sinica  No. 128, Section 2, Academia Road Taipei 115 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chwan\u2010Deng","family":"Hsiao","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology Academia Sinica  No. 128, Section 2, Academia Road Taipei 115 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Lin","family":"Chyan","sequence":"additional","affiliation":[{"name":"Department of Chemistry National Dong Hwa University  No. 1, Section 2, Da Hsueh Road, Shoufeng Hualien 974 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8797-729X","authenticated-orcid":false,"given":"Shang\u2010Cheng","family":"Hung","sequence":"additional","affiliation":[{"name":"Genomics Research Center Academia Sinica  No. 128, Section 2, Academia Road Taipei 115 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,15]]},"reference":[{"key":"e_1_2_2_1_1","unstructured":"World Health Organization (WHO) Global Tuberculosis Report 20th ed. WHO Press Geneva 2015."},{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09657"},{"key":"e_1_2_2_3_1","unstructured":"\u00a0"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/35084083"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2010.08.005"},{"key":"e_1_2_2_6_1","unstructured":"\u00a0"},{"key":"e_1_2_2_7_2","doi-asserted-by":"crossref","first-page":"7464","DOI":"10.1128\/JB.181.24.7464-7469.1999","volume":"181","author":"Delogu G.","year":"1999","journal-title":"J. Bacteriol."},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032421"},{"key":"e_1_2_2_9_1","unstructured":"\u00a0"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01231-10"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2012.01.047"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060713-035314"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2013.10.008"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2008.00741.x"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.03.023"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02206.x"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja302640p"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1073"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/C1OB06415K"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol035606h"},{"key":"e_1_2_2_21_1","unstructured":"\u00a0"},{"key":"e_1_2_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh371"},{"key":"e_1_2_2_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20853"},{"key":"e_1_2_2_24_1","unstructured":"The complete1H 13C and15N chemical-shift assignments the free and bound HBHA110-199have been deposited in the Biological Magnetic Resonance Bank (BMRB) with accession numbers 26887 and 26888 respectively."},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00175245"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10858-015-9924-9"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep29602"},{"key":"e_1_2_2_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2007.01.003"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp508045n"},{"key":"e_1_2_2_30_1","unstructured":"\u00a0"},{"key":"e_1_2_2_31_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20060656"},{"key":"e_1_2_2_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja8026345"},{"key":"e_1_2_2_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201101000"},{"key":"e_1_2_2_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201300313"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fanie.201612518","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.201612518","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T11:49:32Z","timestamp":1694864972000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.201612518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,15]]},"references-count":34,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2017,4,3]]}},"alternative-id":["10.1002\/anie.201612518"],"URL":"https:\/\/doi.org\/10.1002\/anie.201612518","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,15]]}}}