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We present the first synthesis of a previously proposed unsaturated compound that is confirmed to be present in culture extracts of <jats:italic>P<\/jats:italic>.\u2005<jats:italic>aeruginosa<\/jats:italic>, and its structure is shown to be trans\u2010\u0394<jats:sup>1<\/jats:sup>\u20102\u2010(non\u20101\u2010enyl)\u20104\u2010quinolone <jats:italic>N<\/jats:italic>\u2010oxide. This compound is the most active agent against <jats:italic>S.\u2005 aureus<\/jats:italic>, including MRSA strains, by more than one order of magnitude whereas its <jats:italic>cis<\/jats:italic> isomer is inactive. At lower concentrations, the compound induces small\u2010colony variants of <jats:italic>S<\/jats:italic>.\u2005<jats:italic>aureus<\/jats:italic>, reduces the virulence by inhibiting hemolysis, and inhibits nitrate reductase activity under anaerobic conditions. These studies suggest that this unsaturated AQNO is one of the major agents that are used by <jats:italic>P<\/jats:italic>.\u2005<jats:italic>aeruginosa<\/jats:italic> to modulate competing bacterial species.<\/jats:p>","DOI":"10.1002\/anie.201702944","type":"journal-article","created":{"date-parts":[[2017,5,19]],"date-time":"2017-05-19T07:13:33Z","timestamp":1495178013000},"page":"7271-7275","source":"Crossref","is-referenced-by-count":57,"title":["An Unsaturated Quinolone <i>N<\/i>\u2010Oxide of <i>Pseudomonas aeruginosa<\/i> Modulates Growth and Virulence of <i>Staphylococcus aureus<\/i>"],"prefix":"10.1002","volume":"56","author":[{"given":"D\u00e1vid","family":"Szamosv\u00e1ri","sequence":"first","affiliation":[{"name":"Fachbereich Chemie, Konstanz Research School Chemical Biology, Zukunftskolleg Universit\u00e4t Konstanz  78457 Konstanz 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