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These studies allowed us to assign the functions for all four DHs, identifying DH10 as the dedicated dehydratase that catalyzes the dehydration of the C17 hydroxy group during iso\u2010MGS biosynthesis. In contrast to canonical DHs that catalyze dehydration of the \u03b2\u2010hydroxy groups of the nascent polyketide intermediates, DH10 acts in a long\u2010range manner that is unprecedented for type I PKSs, a novel dehydration mechanism that could be exploited for polyketide structural diversity by combinatorial biosynthesis and synthetic biology.<\/jats:p>","DOI":"10.1002\/anie.201703588","type":"journal-article","created":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T17:39:02Z","timestamp":1493746742000},"page":"7247-7251","source":"Crossref","is-referenced-by-count":19,"title":["A Long\u2010Range Acting Dehydratase Domain as the Missing Link for C17\u2010Dehydration in Iso\u2010Migrastatin Biosynthesis"],"prefix":"10.1002","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8241-0630","authenticated-orcid":false,"given":"Bo","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Chemistry The Scripps Research Institute  Jupiter FL 33458 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