{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:30:31Z","timestamp":1790811031034,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"33","license":[{"start":{"date-parts":[[2017,7,17]],"date-time":"2017-07-17T00:00:00Z","timestamp":1500249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001671","name":"European Synchrotron Radiation Facility","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001671","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001721","name":"Rijksuniversiteit Groningen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001721","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2017,8,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tyrosinase\u2010related protein\u20051 (TYRP1) is one of three tyrosinase\u2010like glycoenzymes in human melanocytes that are key to the production of melanin, the compound responsible for the pigmentation of skin, eye, and hair. Difficulties with producing these enzymes in pure form have hampered the understanding of their activity and the effect of mutations that cause albinism and pigmentation disorders. Herein we show that the typical tyrosinase\u2010like subdomain of TYRP1 contains two zinc ions in the active site instead of copper ions as found in tyrosinases, which explains why TYRP1 does not exhibit tyrosinase redox activity. In addition, the structures reveal for the first time that the Cys\u2010rich subdomain, which is unique to vertebrate melanogenic proteins, has an epidermal growth factor\u2010like fold and is tightly associated with the tyrosinase subdomain. Our structures suggest that most albinism\u2010related mutations of TYRP1 affect its stability or activity.<\/jats:p>","DOI":"10.1002\/anie.201704616","type":"journal-article","created":{"date-parts":[[2017,6,29]],"date-time":"2017-06-29T10:06:37Z","timestamp":1498730797000},"page":"9812-9815","source":"Crossref","is-referenced-by-count":224,"title":["Structure of Human Tyrosinase Related Protein\u20051 Reveals a Binuclear Zinc Active Site Important for Melanogenesis"],"prefix":"10.1002","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4989-2444","authenticated-orcid":false,"given":"Xuelei","family":"Lai","sequence":"first","affiliation":[{"name":"Laboratory of Biophysical Chemistry University of Groningen  Nijenborgh 7 9747 AG Groningen The Netherlands"},{"name":"Structural Biology Group European Synchrotron Radiation Facility  71 Avenue des Martyrs 38000 Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry J.","family":"Wichers","sequence":"additional","affiliation":[{"name":"Wageningen Food &amp; Biobased Research  Bornse Weilanden 9 6708 WG Wageningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5924-4734","authenticated-orcid":false,"given":"Montserrat","family":"Soler\u2010Lopez","sequence":"additional","affiliation":[{"name":"Structural Biology Group European Synchrotron Radiation Facility  71 Avenue des Martyrs 38000 Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9731-6586","authenticated-orcid":false,"given":"Bauke W.","family":"Dijkstra","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysical Chemistry University of Groningen  Nijenborgh 7 9747 AG Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,17]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.37.110801.143233"},{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a017046"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06925.x"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.1998.tb00324.x"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2011.01.006"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21332"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-1241"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0161697"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2010.10.048"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102874200"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05083.x"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.8007"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2001.140603.x"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep34993"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/gm13"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-0791"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1217849"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.18.12780"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fanie.201704616","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.201704616","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.201704616","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.201704616","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T10:30:28Z","timestamp":1694341828000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.201704616"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,17]]},"references-count":18,"journal-issue":{"issue":"33","published-print":{"date-parts":[[2017,8,7]]}},"alternative-id":["10.1002\/anie.201704616"],"URL":"https:\/\/doi.org\/10.1002\/anie.201704616","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,17]]}}}