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This includes the use of naturally derived cell membranes, which can bestow nanocarriers with cell\u2010specific functionalities. Herein, we report on a strategy enabling efficient encapsulation of drugs via remote loading into membrane vesicles derived from red blood cells. This is accomplished by supplementing the membrane with additional cholesterol, stabilizing the nanostructure and facilitating the retention of a pH gradient. We demonstrate the loading of two model drugs: the chemotherapeutic doxorubicin and the antibiotic vancomycin. The therapeutic implications of these natural, remote\u2010loaded nanoformulations are studied both in vitro and in vivo using animal disease models. Ultimately, this approach could be used to design new biomimetic nanoformulations with higher efficacy and improved safety profiles.<\/jats:p>","DOI":"10.1002\/anie.201707598","type":"journal-article","created":{"date-parts":[[2017,9,11]],"date-time":"2017-09-11T19:16:34Z","timestamp":1505157394000},"page":"14075-14079","source":"Crossref","is-referenced-by-count":104,"title":["Remote Loading of Small\u2010Molecule Therapeutics into Cholesterol\u2010Enriched Cell\u2010Membrane\u2010Derived Vesicles"],"prefix":"10.1002","volume":"56","author":[{"given":"Xinxin","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of NanoEngineering and Moores Cancer Center University of California, San Diego  La Jolla CA 92093 USA"},{"name":"Shanghai Institute of Materia Medica Chinese Academy of Science  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavimol","family":"Angsantikul","sequence":"additional","affiliation":[{"name":"Department of 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