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The chemical conjugation of a weak antigen to protein as a source of T\u2010cell epitopes generates a glycoconjugate vaccine that results more immunogenic. Several methods have been used so far to characterize the resulting polysaccharide\u2013protein conjugates. However, a reduced number of methodologies has been proposed for measuring the degree of saccharide conjugation at the possible protein sites. Here we show that detailed information on large proteins conjugated with large polysaccharides can be achieved by a combination of solution and solid\u2010state NMR spectroscopy. As a test case, a large protein assembly, <jats:sc>l<\/jats:sc>\u2010asparaginase II, has been conjugated with <jats:italic>Neisseria meningitidis<\/jats:italic> serogroup C capsular polysaccharide and the pattern and degree of conjugation were determined.<\/jats:p>","DOI":"10.1002\/anie.201709274","type":"journal-article","created":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T12:07:29Z","timestamp":1507637249000},"page":"14997-15001","source":"Crossref","is-referenced-by-count":21,"title":["Characterization of the Conjugation Pattern in Large Polysaccharide\u2013Protein Conjugates by NMR Spectroscopy"],"prefix":"10.1002","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2292-8163","authenticated-orcid":false,"given":"Stefano","family":"Giuntini","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Florence  Via della Lastruccia 3 50019 Sesto Fiorentino Italy"},{"name":"Magnetic Resonance Center (CERM) University of Florence and Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine (CIRMMP)  Via L. 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