{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:13:17Z","timestamp":1790244797318,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"44","license":[{"start":{"date-parts":[[2018,10,2]],"date-time":"2018-10-02T00:00:00Z","timestamp":1538438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2018,10,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Bacillus anthracis<\/jats:italic>\n                    owes its pronounced virulence\u2014apart from specific toxins\u2014to a twofold import mechanism for Fe\n                    <jats:sup>III<\/jats:sup>\n                    ions. This pathogenic bacterium secretes the siderophores bacillibactin (BB) and petrobactin (PB), of which only BB is neutralized by human siderocalin, an abundant lipocalin in plasma. We describe its reshaping via combinatorial protein design to bind PB\u22c5Fe\n                    <jats:sup>III<\/jats:sup>\n                    instead of BB\u22c5Fe\n                    <jats:sup>III<\/jats:sup>\n                    , and with even higher affinity (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>D<\/jats:sub>\n                    \u224820\u2005p\n                    <jats:sc>m<\/jats:sc>\n                    ). X\u2010ray crystallographic analysis of the resulting \u201cpetrocalin\u201d in complex with PB\u22c5Ga\n                    <jats:sup>III<\/jats:sup>\n                    reveals a positively charged ligand pocket while the extended butterfly\u2010like conformation of the bound PB provides a rationale for the missing recognition by the natural siderocalin. In microbiological studies, a combination of petrocalin and siderocalin effectively suppressed the growth of a BB\n                    <jats:sup>+<\/jats:sup>\n                    \/PB\n                    <jats:sup>+<\/jats:sup>\n                    strain of\n                    <jats:italic>Bacillus cereus<\/jats:italic>\n                    under iron\u2010limiting culture conditions. Thus, our reprogrammed lipocalin may offer novel treatment options for devastating infections caused by\n                    <jats:italic>B. anthracis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/anie.201807442","type":"journal-article","created":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T08:13:06Z","timestamp":1533111186000},"page":"14619-14623","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Reprogramming Human Siderocalin To Neutralize Petrobactin, the Essential Iron Scavenger of Anthrax Bacillus"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2880-1160","authenticated-orcid":false,"given":"Martin","family":"Dauner","sequence":"first","affiliation":[{"name":"Lehrstuhl f\u00fcr Biologische Chemie Technische Universit\u00e4t M\u00fcnchen  Emil-Erlenmeyer-Forum 5 85354 Freising 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Universit\u00e4t M\u00fcnchen  Weihenstephaner Berg 3 85354 Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5717-498X","authenticated-orcid":false,"given":"Arne","family":"Skerra","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Biologische Chemie Technische Universit\u00e4t M\u00fcnchen  Emil-Erlenmeyer-Forum 5 85354 Freising 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