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Five crystal structures were solved of subfamily 1 and 2 Stig cyclases, which adopt a \u03b2\u2010sandwich fold like the non\u2010catalytic carbohydrate\u2010binding motif. Several complex structures were also determined of indole\u2010based compounds, which are bound to the hydrophobic terminal cavity, where a conserved Asp residue makes an H\u2010bond to the indole N and triggers the acid\u2010catalyzed Cope rearrangement. Through analyzing the enzyme\u2013ligand interactions and mutagenesis experiments, several aromatic residues were found important in catalysis. Apart from a common substrate binding mode and catalytic mechanism, potential subfamily variations that may attribute to the different product specificity are implicated. These results shall expand our scope of enzymology, in particular for further investigation of the biosynthetic Cope rearrangement.<\/jats:p>","DOI":"10.1002\/anie.201808231","type":"journal-article","created":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T15:13:47Z","timestamp":1537197227000},"page":"15060-15064","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The Crystal Structure of a Class of Cyclases that Catalyze the Cope Rearrangement"],"prefix":"10.1002","volume":"57","author":[{"given":"Chun\u2010Chi","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biocatalysis and Enzyme Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province Hubei Key Laboratory of Industrial Biotechnology College of Life Sciences Hubei University  Wuhan 430062 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangying","family":"Hu","sequence":"additional","affiliation":[{"name":"Industrial Enzymes National Engineering Laboratory Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  Tianjin 300308 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueke","family":"Tang","sequence":"additional","affiliation":[{"name":"Industrial Enzymes National Engineering Laboratory Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  Tianjin 300308 China"},{"name":"School of Life Sciences University of Science and Technology of China  Anhui 230026 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunyun","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences; 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