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Although their mode of action is not fully resolved, it is generally accepted that these antimicrobials perturb the structural integrity of the plasma membrane leading to the microbial cell disruption. Here we report on the development of inherently fluorescent antifungal cationic amphiphiles and on the study of their effects on cells of <jats:italic>Candida<\/jats:italic>, one of the most common fungal pathogens in humans. Fluorescent images of <jats:italic>Candida<\/jats:italic> yeast cells that express a fluorescent reporter protein revealed that the cationic amphiphiles rapidly accumulated in the cytosol and led to structural changes in proteins and DNA. Using fluorescent organelle\u2010specific dyes, we showed that these antifungal agents also caused organelle disassembly in <jats:italic>Candida<\/jats:italic> cells. The results of this study indicate that, in designing antifungal cationic amphiphiles for clinical use, the intracellular activities of these molecules must be addressed to avoid undesired side effects to mammalian cells.<\/jats:p>","DOI":"10.1002\/anie.201809410","type":"journal-article","created":{"date-parts":[[2018,10,11]],"date-time":"2018-10-11T13:52:23Z","timestamp":1539265943000},"page":"16391-16395","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Cationic Amphiphiles Induce Macromolecule Denaturation and Organelle Decomposition in Pathogenic Yeast"],"prefix":"10.1002","volume":"57","author":[{"given":"Qais Z.","family":"Jaber","sequence":"first","affiliation":[{"name":"School of Chemistry Raymond and Beverly Sackler Faculty of Exact Sciences Tel Aviv University  Tel Aviv 6997801 Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raphael I.","family":"Benhamou","sequence":"additional","affiliation":[{"name":"School of Chemistry Raymond and Beverly Sackler Faculty of Exact Sciences Tel Aviv University  Tel Aviv 6997801 Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ido M.","family":"Herzog","sequence":"additional","affiliation":[{"name":"School of Chemistry Raymond and Beverly Sackler Faculty of Exact Sciences Tel Aviv University  Tel Aviv 6997801 Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bar","family":"Ben\u2005Baruch","sequence":"additional","affiliation":[{"name":"Department of Neurobiology George S. 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