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A critical barrier to understanding the biology of HNO and furthering clinical development is the quantification and real\u2010time monitoring of its delivery in living systems. Herein, we describe the design and synthesis of the first chemiluminescent probe for HNO, <jats:bold>HNOCL\u20101<\/jats:bold>, which can detect HNO generated from concentrations of Angeli's salt as low as 138\u2005n<jats:sc>m<\/jats:sc> with high selectivity based on the reaction with a phosphine group to form a self\u2010cleavable azaylide intermediate. We have capitalized on this high sensitivity to develop a generalizable kinetics\u2010based approach, which provides real\u2010time quantitative measurements of HNO concentration at the picomolar level. <jats:bold>HNOCL\u20101<\/jats:bold> can monitor dynamics of HNO delivery in living cells and tissues, demonstrating the versatility of this method for tracking HNO in living systems.<\/jats:p>","DOI":"10.1002\/anie.201811257","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T18:06:24Z","timestamp":1543255584000},"page":"1361-1365","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":113,"title":["A Chemiluminescent Probe for HNO Quantification and Real\u2010Time Monitoring in Living Cells"],"prefix":"10.1002","volume":"58","author":[{"given":"Weiwei","family":"An","sequence":"first","affiliation":[{"name":"Department of Chemistry Center for Drug Discovery, Design, and Delivery (CD4), and Center 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