{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:58:43Z","timestamp":1790783923526,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T00:00:00Z","timestamp":1551830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["14420"],"award-info":[{"award-number":["14420"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2019,4,16]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We show herein that allostery offers a key strategy for the design of out\u2010of\u2010equilibrium systems by engineering allosteric DNA\u2010based nanodevices for the transient loading and release of small organic molecules. To demonstrate the generality of our approach, we used two model DNA\u2010based aptamers that bind ATP and cocaine through a target\u2010induced conformational change. We re\u2010engineered these aptamers so that their affinity towards their specific target is controlled by a DNA sequence acting as an allosteric inhibitor. The use of an enzyme that specifically cleaves the inhibitor only when it is bound to the aptamer generates a transient allosteric control that leads to the release of ATP or cocaine from the aptamers. Our approach confirms that the programmability and predictability of nucleic acids make synthetic DNA\/RNA the perfect candidate material to re\u2010engineer synthetic receptors that can undergo chemical fuel\u2010triggered release of small\u2010molecule cargoes and to rationally design non\u2010equilibrium systems.<\/jats:p>","DOI":"10.1002\/anie.201812885","type":"journal-article","created":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T00:49:09Z","timestamp":1549327749000},"page":"5582-5586","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":114,"title":["Fuel\u2010Responsive Allosteric DNA\u2010Based Aptamers for the Transient Release of ATP and Cocaine"],"prefix":"10.1002","volume":"58","author":[{"given":"Erica","family":"Del\u2005Grosso","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Chimiche University of Rome Tor Vergata  Via della Ricerca Scientifica Rome 00133 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Ragazzon","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences University of Padua  Via Marzolo 1 35131 Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard J.","family":"Prins","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences University of Padua  Via Marzolo 1 35131 Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4941-8646","authenticated-orcid":false,"given":"Francesco","family":"Ricci","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Chimiche University of Rome Tor Vergata  Via della Ricerca Scientifica Rome 00133 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,6]]},"reference":[{"key":"e_1_2_2_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2016.116"},{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6CS00738D"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-018-0192-1"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201001511"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201001511"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4SC01258E"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac6103"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2511"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.5b00279"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201610946"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201610946"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b07469"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aao1377"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15895"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15899"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03542-z"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8790"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201711964"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201711964"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201801318"},{"key":"e_1_2_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201801318"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8790"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CS00121E"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201706750"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-018-0250-8"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201810891"},{"key":"e_1_2_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201810891"},{"key":"e_1_2_2_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CS00245A"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biophys-083012-130257"},{"key":"e_1_2_2_25_1","volume-title":"Mechanisms of Cooperativity and Allosteric Regulation in Proteins","author":"Perutz M.","year":"1990"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr0306743"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3141"},{"key":"e_1_2_2_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi047507x"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.609"},{"key":"e_1_2_2_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja032013t"},{"key":"e_1_2_2_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja310585e"},{"key":"e_1_2_2_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200805819"},{"key":"e_1_2_2_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200805819"},{"key":"e_1_2_2_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00002a033"},{"key":"e_1_2_2_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx517"},{"key":"e_1_2_2_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0038171"},{"key":"e_1_2_2_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn403772j"},{"key":"e_1_2_2_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr400354z"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fanie.201812885","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.201812885","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.201812885","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.201812885","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T05:07:08Z","timestamp":1693976828000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.201812885"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,6]]},"references-count":43,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2019,4,16]]}},"alternative-id":["10.1002\/anie.201812885"],"URL":"https:\/\/doi.org\/10.1002\/anie.201812885","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,6]]},"assertion":[{"value":"2018-11-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}