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The deconvolution of their targets via chemical proteomics is often challenged by the introduction of large photocrosslinkers. Here we applied elegaphenone, a largely uncharacterized natural product antibiotic bearing a native benzophenone core scaffold, for affinity\u2010based protein profiling (AfBPP) in Gram\u2010positive and Gram\u2010negative bacteria. This study utilizes the alkynylated natural product scaffold as a probe to uncover intriguing biological interactions with the transcriptional regulator AlgP. Furthermore, proteome profiling of a Pseudomonas aeruginosa AlgP transposon mutant provided unique insights into the mode of action. Elegaphenone enhanced the elimination of intracellular <jats:italic>P.\u2005aeruginosa<\/jats:italic> in macrophages exposed to sub\u2010inhibitory concentrations of the fluoroquinolone antibiotic norfloxacin.<\/jats:p>","DOI":"10.1002\/anie.201903472","type":"journal-article","created":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T15:33:04Z","timestamp":1554910384000},"page":"8581-8584","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The Natural Product Elegaphenone Potentiates Antibiotic Effects against <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1002","volume":"58","author":[{"given":"Weining","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Chemistry Center for Integrated Protein Science Munich (CIPSM) Technische Universit\u00e4t M\u00fcnchen  Lichtenbergstra\u00dfe 4 85747 Garching Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley 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