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This method involves local sampling using hydrodynamic flow confinement of a lysis buffer, followed by electrokinetic purification of nucleic acids from the sampled lysate. We characterized the method by locally sampling an array of points with a circa 200\u2005\u03bcm diameter footprint, enabling the detection of single <jats:italic>KRAS<\/jats:italic> and <jats:italic>BRAF<\/jats:italic> point mutations in small populations of RKO and MCF\u20107 FFPE cell blocks. To illustrate the utility of this approach for genetic analysis, we demonstrate spatially resolved genotyping of FFPE sections of human breast invasive ductal carcinoma.<\/jats:p>","DOI":"10.1002\/anie.201907150","type":"journal-article","created":{"date-parts":[[2019,9,17]],"date-time":"2019-09-17T06:50:46Z","timestamp":1568703046000},"page":"15259-15262","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Spatially Resolved Genetic Analysis of Tissue Sections Enabled by Microscale Flow Confinement Retrieval and Isotachophoretic Purification"],"prefix":"10.1002","volume":"58","author":[{"given":"Xander F.","family":"van Kooten","sequence":"first","affiliation":[{"name":"IBM Research\u2014Zurich  S\u00e4umerstrasse 4 8803 R\u00fcschlikon Switzerland"},{"name":"Faculty of Mechanical Engineering, Technion\u2014 Israel Institute of Technology  Technion City 3200003 Haifa Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenzo F. 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