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Here, we describe a novel class of butenolides isolated from a Gram\u2010negative <jats:italic>Pseudomonas<\/jats:italic> strain, the styrolides. Structure elucidation was aided by the total synthesis of styrolide\u2005A. Transposon mutagenesis enabled us to identify the styrolide biosynthetic gene cluster, and by using a homology search, we discovered the related and previously unknown acaterin biosynthetic gene cluster in another <jats:italic>Pseudomonas<\/jats:italic> species. Mutagenesis, heterologous expression, and identification of key shunt and intermediate products were crucial to propose a biosynthetic pathway for both <jats:italic>Pseudomonas<\/jats:italic>\u2010derived butenolides. 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