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Adechlorin (ade) is a 2\u2032\u2010chloro nucleoside natural product first identified from <jats:italic>Actinomadura<\/jats:italic> sp. ATCC 39365. However, the installation of chlorine in the ade biosynthetic pathway remains elusive. Reported herein is a Fe<jats:sup>2+<\/jats:sup>\u2010\u03b1\u2010ketoglutarate halogenase AdeV that can install a chlorine atom at the C2\u2032 position of 2\u2032\u2010deoxyadenosine monophosphate to afford 2\u2032\u2010chloro\u20102\u2032\u2010deoxyadenosine monophosphate. Furthermore, 2\u2032,3\u2032\u2010dideoxyadenosine\u20105\u2032\u2010monophosphate and 2\u2032\u2010deoxyinosine\u20105\u2032\u2010monophosphate can also be converted, albeit 20\u2010fold and 2\u2010fold, respectively, less efficiently relative to the conversion of 2\u2032\u2010deoxyadenosine monophosphate. AdeV represents the first example of a Fe<jats:sup>2+<\/jats:sup>\u2010\u03b1\u2010ketoglutarate\u2010dependent halogenase that converts nucleotides into chlorinated analogues.<\/jats:p>","DOI":"10.1002\/anie.201914994","type":"journal-article","created":{"date-parts":[[2020,3,11]],"date-time":"2020-03-11T17:17:50Z","timestamp":1583947070000},"page":"9478-9484","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["An Fe<sup>2+<\/sup>\u2010 and \u03b1\u2010Ketoglutarate\u2010Dependent Halogenase Acts on Nucleotide Substrates"],"prefix":"10.1002","volume":"59","author":[{"given":"Chunhua","family":"Zhao","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation School of Pharmacy Tongji Medical College Huazhong University of Science and Technology  Wuhan 430030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Yan","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation School of Pharmacy Tongji Medical College Huazhong University of Science and Technology  Wuhan 430030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation School of Pharmacy Tongji Medical College Huazhong University of Science and Technology  Wuhan 430030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hucheng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation School of Pharmacy Tongji Medical College Huazhong University of Science and Technology  Wuhan 430030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyu","family":"Zhong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education School of Pharmaceutical Sciences Wuhan University  Wuhan 430071 P. 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