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Herein, we reductively cage the fluorescent DNA probes conjugated with a cyanine dye to hydrocyanine, acting as a photoactivatable dark state. The additional dark state from caging lowered the fluorescent background while enabling optically selective activation by total internal reflection (TIR) illumination at 405\u2005nm. These benefits from \u201creductive caging\u201d helped to increase the localization density or the imaging speed while preserving the image quality. With the aid of high\u2010density analysis, we could further increase the imaging speed of conventional DNA\u2010PAINT by two orders of magnitude, making DNA\u2010PAINT capable of high\u2010throughput super\u2010resolution imaging.<\/jats:p>","DOI":"10.1002\/anie.201915377","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T04:14:34Z","timestamp":1587442474000},"page":"11758-11762","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Reductively Caged, Photoactivatable DNA\u2010PAINT for High\u2010Throughput Super\u2010resolution Microscopy"],"prefix":"10.1002","volume":"59","author":[{"given":"Soohyun","family":"Jang","sequence":"first","affiliation":[{"name":"Center for Molecular Spectroscopy and Dynamics Institute for Basic Science (IBS)  Anam-ro 145, Sungbuk-gu Seoul 02841 Republic of Korea"},{"name":"Department of Chemistry Korea University  Anam-ro 145, Sungbuk-gu Seoul 02841 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry Korea University  Anam-ro 145, Sungbuk-gu Seoul 02841 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9964-7231","authenticated-orcid":false,"given":"Sang\u2010Hee","family":"Shim","sequence":"additional","affiliation":[{"name":"Center for Molecular Spectroscopy and Dynamics Institute for Basic Science (IBS)  Anam-ro 145, Sungbuk-gu Seoul 02841 Republic of Korea"},{"name":"Department of Chemistry Korea University  Anam-ro 145, Sungbuk-gu Seoul 02841 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,18]]},"reference":[{"key":"e_1_2_2_1_1","unstructured":"\u00a0"},{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1127344"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.091116"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth929"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609643104"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl103427w"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2835"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-018-0105-0"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201905685"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201905685"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2016.95"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.024"},{"key":"e_1_2_2_12_1","first-page":"359","volume":"13","author":"Legant W. 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