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Our strategy allows the facile preparation of spin\u2010labeled RNAs with sizes ranging from short RNA oligonucleotides to large, complex RNA molecules with over 370 nucleotides by standard in\u2005vitro transcription. As a proof of concept, inter\u2010spin distance distributions are measured by pulsed electron paramagnetic resonance (EPR) spectroscopy in short self\u2010complementary RNA sequences and in a well\u2010studied 185\u2005nucleotide non\u2010coding RNA, the\n                    <jats:italic>B.\u2005subtilis glmS<\/jats:italic>\n                    ribozyme. 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