{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:29:03Z","timestamp":1786976943948,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["682586"],"award-info":[{"award-number":["682586"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2020,6,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In\u2005vitro selected ribozymes are promising tools for site\u2010specific labeling of RNA. Previously known nucleic acid catalysts attached fluorescently labeled adenosine or guanosine derivatives through 2\u2032,5\u2032\u2010branched phosphodiester bonds to the RNA of interest. Herein, we report new ribozymes that use orthogonal substrates, derived from the antiviral drug tenofovir, and attach bioorthogonal functional groups, as well as affinity handles and fluorescent reporter units through a hydrolytically more stable phosphonate ester linkage. The tenofovir transferase ribozymes were identified by in\u2005vitro selection and are orthogonal to nucleotide transferase ribozymes. As genetically encodable functional RNAs, these ribozymes may be developed for potential cellular applications. The orthogonal ribozymes addressed desired target sites in large RNAs in\u2005vitro, as shown by fluorescent labeling of\n                    <jats:italic>E.\u2005coli<\/jats:italic>\n                    16S and 23S rRNAs in total cellular RNA.\n                  <\/jats:p>","DOI":"10.1002\/anie.202001300","type":"journal-article","created":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T01:45:27Z","timestamp":1583977527000},"page":"9335-9339","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Repurposing Antiviral Drugs for Orthogonal RNA\u2010Catalyzed Labeling of RNA"],"prefix":"10.1002","volume":"59","author":[{"given":"Mohammad","family":"Ghaem Maghami","sequence":"first","affiliation":[{"name":"Universit\u00e4t W\u00fcrzburg Institut f\u00fcr Organische Chemie  Am Hubland 97074 W\u00fcrzburg Germany"},{"name":"International Max Planck Research School Molecular Biology University of G\u00f6ttingen  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surjendu","family":"Dey","sequence":"additional","affiliation":[{"name":"Universit\u00e4t W\u00fcrzburg Institut f\u00fcr Organische Chemie  Am Hubland 97074 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann\u2010Kathrin","family":"Lenz","sequence":"additional","affiliation":[{"name":"Universit\u00e4t W\u00fcrzburg Institut f\u00fcr Organische Chemie  Am Hubland 97074 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4548-2299","authenticated-orcid":false,"given":"Claudia","family":"H\u00f6bartner","sequence":"additional","affiliation":[{"name":"Universit\u00e4t W\u00fcrzburg Institut f\u00fcr Organische Chemie  Am Hubland 97074 W\u00fcrzburg Germany"},{"name":"International Max Planck Research School Molecular Biology University of G\u00f6ttingen  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4]]},"reference":[{"key":"e_1_2_2_1_1","unstructured":"\u00a0"},{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2015.11.016"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2019.1593093"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583519000064"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1561"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky513"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201302874"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201302874"},{"key":"e_1_2_2_8_1","unstructured":"\u00a0"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks381"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b07286"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201410433"},{"key":"e_1_2_2_11_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201410433"},{"key":"e_1_2_2_12_1","unstructured":"\u00a0"},{"key":"e_1_2_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200250611"},{"key":"e_1_2_2_13_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200250611"},{"key":"e_1_2_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja503864v"},{"key":"e_1_2_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2013.04.007"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b10531"},{"key":"e_1_2_2_17_1","unstructured":"\u00a0"},{"key":"e_1_2_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201200091"},{"key":"e_1_2_2_19_2","first-page":"226","volume":"4","author":"Martin G.","year":"1998","journal-title":"RNA"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CC06703H"},{"key":"e_1_2_2_21_1","unstructured":"\u00a0"},{"key":"e_1_2_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1877"},{"key":"e_1_2_2_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr5002035"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1086\/378068"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2015.11.009"},{"key":"e_1_2_2_26_1","unstructured":"\u00a0"},{"key":"e_1_2_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.7.1568"},{"key":"e_1_2_2_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106324"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-3542(01)00210-8"},{"key":"e_1_2_2_30_1","doi-asserted-by":"publisher","DOI":"10.1135\/cccc20011545"},{"key":"e_1_2_2_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/mtna.2015.4"},{"key":"e_1_2_2_32_1","unstructured":"FJ is our laboratory nomenclature for the tenofovir transferase ribozyme selection and the numbers 1 and 8 relate to arbitrary clone numbers for Sanger sequencing. C in FJC refers to sequence cluster identified in NGS data; C9 is the 9th cluster in the output generated by fastaptamer_cluster. See the Supporting Information for more details."},{"key":"e_1_2_2_33_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.068213.118"},{"key":"e_1_2_2_34_1","unstructured":"The tenofovir analogues synthesized in this study were used as racemic mixtures. The comparison with commercially availableN6-unmodified (R)-tenofovir diphosphate revealed similar activity of FJ1 with enantiomerically pure and racemic tenofovir diphosphate substrate (Figure\u2005S15)."}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fanie.202001300","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202001300","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.202001300","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202001300","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T10:06:22Z","timestamp":1693908382000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.202001300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4]]},"references-count":37,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2020,6,8]]}},"alternative-id":["10.1002\/anie.202001300"],"URL":"https:\/\/doi.org\/10.1002\/anie.202001300","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4]]},"assertion":[{"value":"2020-01-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}