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Detection tools for biological FA are therefore of great interest. Reported here are novel activity\u2010based genetically encoded fluorescent and luminescent probes for detecting FA in aqueous solutions and living mammalian cells. A FA\u2010reactive lysine analogue, PrAK, was site\u2010specifically incorporated into the essential lysine sites of enhanced green fluorescent protein (EGFP) and firefly luciferase (fLuc) to afford fluorescent and luminescent FA probes, respectively. FA selectively reacts with PrAK residues on EGFP and fLuc through a 2\u2010aza\u2010Cope rearrangement, resulting in fluorescence and luminescence turn\u2010on responses, respectively, to FA selectively over potentially interfering reactive species in aqueous buffer. 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