{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:49:48Z","timestamp":1790999388161,"version":"4.1.0"},"reference-count":81,"publisher":"Wiley","issue":"29","license":[{"start":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T00:00:00Z","timestamp":1589760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["VA 824\/1-2"],"award-info":[{"award-number":["VA 824\/1-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2020,7,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Glypiation is a common posttranslational modification of eukaryotic proteins involving the attachment of a glycosylphosphatidylinositol (GPI) glycolipid. GPIs contain a conserved phosphoglycan that is modified in a cell\u2010 and tissue\u2010specific manner. GPI complexity suggests roles in biological processes and effects on the attached protein, but the difficulties to get homogeneous material have hindered studies. We disclose a one\u2010pot intein\u2010mediated ligation (OPL) to obtain GPI\u2010anchored proteins. The strategy enables the glypiation of folded and denatured proteins with a natural linkage to the glycolipid. Using the strategy, glypiated eGFP, Thy1, and the\n                    <jats:italic>Plasmodium berghei<\/jats:italic>\n                    protein MSP1\n                    <jats:sub>19<\/jats:sub>\n                    were prepared. Glypiation did not alter the structure of eGFP and MSP1\n                    <jats:sub>19<\/jats:sub>\n                    proteins in solution, but it induced a strong pro\u2010inflammatory response in\u2005vitro. The strategy provides access to glypiated proteins to elucidate the activity of this modification and for use as vaccine candidates against parasitic infections.\n                  <\/jats:p>","DOI":"10.1002\/anie.202002479","type":"journal-article","created":{"date-parts":[[2020,4,19]],"date-time":"2020-04-19T21:50:42Z","timestamp":1587333042000},"page":"12035-12040","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Semisynthesis of Functional Glycosylphosphatidylinositol\u2010Anchored Proteins"],"prefix":"10.1002","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7701-7463","authenticated-orcid":false,"given":"Ren\u00e9e F.","family":"Roller","sequence":"first","affiliation":[{"name":"Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces  14424 Potsdam Germany"},{"name":"Institute of Chemistry and Biochemistry Freie Universit\u00e4t Berlin  Arnimallee 22 14195 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankita","family":"Malik","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces  14424 Potsdam Germany"},{"name":"Institute of Chemistry and Biochemistry Freie Universit\u00e4t Berlin  Arnimallee 22 14195 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A.","family":"Carillo","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces  14424 Potsdam Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Garg","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces  14424 Potsdam Germany"},{"name":"Institute of Chemistry and Biochemistry Freie Universit\u00e4t Berlin  Arnimallee 22 14195 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Rella","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces  14424 Potsdam Germany"},{"name":"Institute of Chemistry and Biochemistry Freie Universit\u00e4t Berlin  Arnimallee 22 14195 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Kristin","family":"Raulf","sequence":"additional","affiliation":[{"name":"Immunology Unit and Research Center for Emerging Infections and Zoonoses University of Veterinary Medicine Hannover  B\u00fcnteweg 17 30559 Hannover Germany"},{"name":"Institute for Parasitology, Center for infection Medicine University of Veterinary Medicine Hannover  B\u00fcnteweg 17 30559 Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Lepenies","sequence":"additional","affiliation":[{"name":"Immunology Unit and Research Center for Emerging Infections and Zoonoses University of Veterinary Medicine Hannover  B\u00fcnteweg 17 30559 Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3394-8466","authenticated-orcid":false,"given":"Peter H.","family":"Seeberger","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces  14424 Potsdam Germany"},{"name":"Institute of Chemistry and Biochemistry Freie Universit\u00e4t Berlin  Arnimallee 22 14195 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2804-1923","authenticated-orcid":false,"given":"Daniel","family":"Var\u00f3n Silva","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces  14424 Potsdam Germany"},{"name":"Institute of Chemistry and Biochemistry Freie Universit\u00e4t Berlin  Arnimallee 22 14195 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,18]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.17.2799"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201203912"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201203912"},{"key":"e_1_2_6_3_1","unstructured":"\u00a0"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.57.070188.001441"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.25.409"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31204-2"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1410150"},{"key":"e_1_2_6_8_1","unstructured":"\u00a0"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwq038"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)30324-6"},{"key":"e_1_2_6_11_1","unstructured":"\u00a0"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.1.416"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.1999.00268.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"5890","DOI":"10.4049\/jimmunol.158.12.5890","volume":"158","author":"Camargo M. M.","year":"1997","journal-title":"J. Immunol."},{"key":"e_1_2_6_15_1","unstructured":"\u00a0"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.09.007"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M509631200"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/11.4.45R"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.02.003"},{"key":"e_1_2_6_20_1","unstructured":"\u00a0"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(95)02518-9"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.11.1563"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200353251"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200353251"},{"key":"e_1_2_6_24_1","unstructured":"\u00a0"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.7b01132"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200802161"},{"key":"e_1_2_6_26_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200802161"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0cc04172f"},{"key":"e_1_2_6_28_1","unstructured":"\u00a0"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0cc00828a"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja906611x"},{"key":"e_1_2_6_31_1","unstructured":"\u00a0"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.02.003"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja309126m"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2sc21515b"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201103483"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201103483"},{"key":"e_1_2_6_36_1","unstructured":"\u00a0"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)80764-8"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(98)01063-1"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/c39760000998"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7973629"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201407160"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201407160"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.2.487"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2865810"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-016-5112-0"},{"key":"e_1_2_6_45_1","unstructured":"\u00a0"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90228-C"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90128-S"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90002-F"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(96)02696-5"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009990515"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003085"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.698282"},{"key":"e_1_2_6_53_1","unstructured":"\u00a0"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M600035-MCP200"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.09.007"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(87)90189-7"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.9.2989"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000053889"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-11-611707"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.51.1.97"},{"key":"e_1_2_6_61_1","unstructured":"See Ref.\u2005[13b]."},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2005.06.005"},{"key":"e_1_2_6_63_1","doi-asserted-by":"crossref","first-page":"5993","DOI":"10.4049\/jimmunol.162.10.5993","volume":"162","author":"Kukulansky T.","year":"1999","journal-title":"J. Immunol."},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1096-1246"},{"key":"e_1_2_6_65_1","unstructured":"\u00a0"},{"key":"e_1_2_6_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057086"},{"key":"e_1_2_6_67_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210716200"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)00128-X"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.8.4022"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.2002.00478.x"},{"key":"e_1_2_6_71_1","unstructured":"\u00a0"},{"key":"e_1_2_6_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.3909-3915.2000"},{"key":"e_1_2_6_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5630-5637.2004"},{"key":"e_1_2_6_74_1","unstructured":"\u00a0"},{"key":"e_1_2_6_75_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.70.4.467"},{"key":"e_1_2_6_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(02)01617-9"}],"container-title":["Angewandte Chemie International Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fanie.202002479","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202002479","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/anie.202002479","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.202002479","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T19:20:03Z","timestamp":1693682403000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.202002479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,18]]},"references-count":81,"journal-issue":{"issue":"29","published-print":{"date-parts":[[2020,7,13]]}},"alternative-id":["10.1002\/anie.202002479"],"URL":"https:\/\/doi.org\/10.1002\/anie.202002479","archive":["Portico"],"relation":{},"ISSN":["1433-7851","1521-3773"],"issn-type":[{"value":"1433-7851","type":"print"},{"value":"1521-3773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,18]]},"assertion":[{"value":"2020-02-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}