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Bacterial culturing is used for <jats:italic>L. pneumophila<\/jats:italic> detection, but this method takes approximately 10 days to complete. In this work, an RNA\u2010cleaving fluorogenic DNAzyme, named LP1, was isolated. Extensive characterization revealed that LP1 is reactive with multiple infectious isolates of <jats:italic>L.\u2005pneumophila<\/jats:italic> but inactive with 25 other common bacterial species. LP1 is likely activated by a protein target, capable of generating a detectable signal in the presence of as few as 10 colony\u2010forming units of <jats:italic>L. pneumophila<\/jats:italic>, and able to maintain its activity in cooling tower water from diverse sources. Given that similar DNAzymes have been incorporated into many sensitive assays for bacterial detection, LP1 holds the potential for the development of biosensors for monitoring the contamination of <jats:italic>L. pneumophila<\/jats:italic> in exposure sources.<\/jats:p>","DOI":"10.1002\/anie.202012444","type":"journal-article","created":{"date-parts":[[2020,11,14]],"date-time":"2020-11-14T03:29:46Z","timestamp":1605324586000},"page":"4782-4788","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Selection and Characterization of an RNA\u2010Cleaving DNAzyme Activated by <i>Legionella pneumophila<\/i>"],"prefix":"10.1002","volume":"60","author":[{"given":"Meghan","family":"Rothenbroker","sequence":"first","affiliation":[{"name":"Michael G. 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