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Natural products containing Dha and\/or Dhb residues are often found to display diverse biological activities. While the (<jats:italic>Z<\/jats:italic>) geometry is predominant in nature, only a handful of metabolites containing (<jats:italic>E<\/jats:italic>)\u2010Dhb have been found thus far. Here we report discovery of a new antimicrobial peptide, albopeptide, through NMR analysis and chemical synthesis, which contains two contiguous unsaturated residues, Dha\u2010(<jats:italic>E<\/jats:italic>)\u2010Dhb. It displays narrow\u2010spectrum activity against vancomycin\u2010resistant <jats:italic>Enterococcus\u2005faecium<\/jats:italic>. In\u2010vitro biochemical assays show that albopeptide originates from a noncanonical NRPS pathway featuring dehydration processes and catalysed by unusual condensation domains. Finally, we provide evidence of the occurrence of a previously untapped group of short unsaturated peptides in the bacterial kingdom, suggesting an important biological function in bacteria.<\/jats:p>","DOI":"10.1002\/anie.202012902","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T15:00:39Z","timestamp":1603810839000},"page":"3229-3237","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Discovery and Biosynthetic Investigation of a New Antibacterial Dehydrated Non\u2010Ribosomal Tripeptide"],"prefix":"10.1002","volume":"60","author":[{"given":"Shan","family":"Wang","sequence":"first","affiliation":[{"name":"Marine Biodiscovery Centre Department of Chemistry University of Aberdeen  Meston Walk Aberdeen AB24 3UE Scotland UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Fang","sequence":"additional","affiliation":[{"name":"Marine Biodiscovery Centre Department of Chemistry University of Aberdeen  Meston Walk Aberdeen AB24 3UE Scotland UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Lu","sequence":"additional","affiliation":[{"name":"Marine Biodiscovery Centre Department of Chemistry University of Aberdeen  Meston Walk Aberdeen AB24 3UE Scotland UK"},{"name":"Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application State Key Laboratory of Applied Microbiology Southern China Guangdong Institute of Microbiology  Guangdong Academy of Sciences China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingli","family":"Gao","sequence":"additional","affiliation":[{"name":"Marine Biodiscovery Centre Department of Chemistry University of Aberdeen  Meston Walk Aberdeen AB24 3UE Scotland UK"},{"name":"College of Marine Life and Fisheries Jiangsu Ocean University  Lianyungang Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Trembleau","sequence":"additional","affiliation":[{"name":"Marine Biodiscovery Centre Department of Chemistry University of Aberdeen  Meston Walk Aberdeen AB24 3UE Scotland UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer","family":"Ebel","sequence":"additional","affiliation":[{"name":"Marine Biodiscovery Centre Department of Chemistry University of Aberdeen  Meston Walk Aberdeen AB24 3UE Scotland UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanette H.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Marbio, UiT\u2014 The Arctic University of Norway  9037 Troms\u00f8 Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Philips","sequence":"additional","affiliation":[{"name":"NCIMB Ltd.  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