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The final images are background\u2010free and show confocality and enhanced spatial resolution (super\u2010resolution). Fluorophore photobleaching, however, is a key limitation for recording long time series of images that will allow for the calculation of higher order SOFI results with correspondingly increased resolution. Here, we demonstrate that photobleaching can be circumvented by using fluorophore labels that reversibly and transiently bind to a target, and which are being replenished from a buffer which serves as a reservoir. Using fluorophore\u2010labeled short DNA oligonucleotides, we labeled cellular structures with target\u2010specific antibodies that contain complementary DNA sequences and record the fluctuation events caused by transient emitter binding. We show that this concept bypasses extensive photobleaching and facilitates two\u2010color imaging of cellular structures with SOFI.<\/jats:p>","DOI":"10.1002\/anie.202013166","type":"journal-article","created":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T04:32:50Z","timestamp":1607661170000},"page":"6310-6313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Multi\u2010Color, Bleaching\u2010Resistant Super\u2010Resolution Optical Fluctuation Imaging with Oligonucleotide\u2010Based Exchangeable Fluorophores"],"prefix":"10.1002","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2834-2785","authenticated-orcid":false,"given":"Marius","family":"Glogger","sequence":"first","affiliation":[{"name":"Institute of Physical and Theoretical Chemistry Goethe-University Frankfurt  Max-von-Laue Str. 7 60438 Frankfurt 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