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Developed here is a CRISPR\/Cas9\u2010mediated triple\u2010line lateral flow assay (TL\u2010LFA) combined with multiplex reverse transcription\u2010recombinase polymerase amplification (RT\u2010RPA) for rapid and simultaneous dual\u2010gene detection of SARS\u2010CoV\u20102 in a single strip test. This assay is characterized by the detection of envelope (E) and open reading frame 1ab (Orf1ab) genes from cell\u2010cultured SARS\u2010CoV\u20102 and SARS\u2010CoV\u20102 viral RNA standards, showing a sensitivity of 100 RNA copies per reaction (25\u2005\u03bcL). Furthermore, dual\u2010gene analysis of 64 nasopharyngeal swab samples showed 100\u2009% negative predictive agreement and 97.14\u2009% positive predictive agreement. This platform will provide a more accurate and convenient pathway for diagnosis of COVID\u201019 or other infectious diseases in low\u2010resource regions.<\/jats:p>","DOI":"10.1002\/anie.202014506","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T01:58:51Z","timestamp":1607565531000},"page":"5307-5315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":288,"title":["Simultaneous Dual\u2010Gene Diagnosis of SARS\u2010CoV\u20102 Based on CRISPR\/Cas9\u2010Mediated Lateral Flow Assay"],"prefix":"10.1002","volume":"60","author":[{"given":"Erhu","family":"Xiong","sequence":"first","affiliation":[{"name":"School of Life Sciences South China Normal University  Guangzhou 510631 China"},{"name":"State Key Laboratory of Chemo\/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University  Changsha 410082 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemo\/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University  Changsha 410082 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Life Sciences South China Normal University  Guangzhou 510631 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menglu","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Life Sciences South China Normal University  Guangzhou 510631 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huahua","family":"Yue","sequence":"additional","affiliation":[{"name":"School of Life Sciences South China Normal University  Guangzhou 510631 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqi","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Life Sciences South China Normal University  Guangzhou 510631 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Life Sciences South China Normal University  Guangzhou 510631 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongzhong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Hubei Provincial Center for Disease Control and Prevention  Wuhan 430079 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine School of Chemistry South China Normal University  Guangzhou 510006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5597-4804","authenticated-orcid":false,"given":"Xiaoming","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Life Sciences South China Normal University  Guangzhou 510631 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,26]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chas.0c00035"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tre.2020.101922"},{"key":"e_1_2_6_3_1","first-page":"1","volume":"29","author":"Xia L. 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