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Here, we report the development of a high\u2010throughput single\u2010particle method to enumerate intact viral particles by ultrasensitive flow virometry, which detects single viruses as small as 27\u2005nm in diameter. The nucleic acid dye SYTO 82 was used to stain the viral (or vector) genome, and a laboratory\u2010built nano\u2010flow cytometer (nFCM) was employed to simultaneously detect the side\u2010scatter and fluorescence signals of individual viral particles. Using the bacteriophage T7 as a model system, intact virions were completely discriminated from empty capsids and naked viral genomes. Successful measurement of the physical virus titer and purity was demonstrated for recombinant adenoviruses, which could be used for gene delivery, therapeutic products derived from phage cocktails, and infected cell supernatants for veterinary vaccine production.<\/jats:p>","DOI":"10.1002\/anie.202100872","type":"journal-article","created":{"date-parts":[[2021,2,16]],"date-time":"2021-02-16T08:55:22Z","timestamp":1613465722000},"page":"9351-9356","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Quantitative Assessment of the Physical Virus Titer and Purity by Ultrasensitive Flow Virometry"],"prefix":"10.1002","volume":"60","author":[{"given":"Qian","family":"Niu","sequence":"first","affiliation":[{"name":"Department of Chemical Biology MOE Key Laboratory of Spectrochemical Analysis &amp; Instrumentation Key Laboratory for Chemical Biology of Fujian Province College of Chemistry and Chemical Engineering Xiamen University  Xiamen 361005 P. 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