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The state\u2010of\u2010the\u2010art techniques to quantify cell forces employ deformable polymer films or molecular probes tethered to glass substrates. However, the applications of these assays in fundamental and clinical research are restricted by the planar geometry and low throughput of microscopy readout. Herein, we develop a DNA\u2010based microparticle tension sensor, which features a spherical surface and thus allows for investigation of mechanotransduction at curved interfaces. The micron\u2010scale of \u03bcTS enables flow cytometry readout, which is rapid and high throughput. We applied the method to map and measure T\u2010cell receptor forces and platelet integrin forces at 12 and 56\u2005pN thresholds. Furthermore, we quantified the inhibition efficiency of two anti\u2010platelet drugs providing a proof\u2010of\u2010concept demonstration of \u03bcTS to screen drugs that modulate cellular mechanics.<\/jats:p>","DOI":"10.1002\/anie.202102206","type":"journal-article","created":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T13:10:23Z","timestamp":1620825023000},"page":"18044-18050","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["DNA\u2010Based Microparticle Tension Sensors (\u03bcTS) for Measuring Cell Mechanics in Non\u2010planar Geometries and for High\u2010Throughput Quantification"],"prefix":"10.1002","volume":"60","author":[{"given":"Yuesong","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Chemistry Emory University  Atlanta GA 30322 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor Pui\u2010Yan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Chemistry Emory University  Atlanta GA 30322 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Chemistry Emory University  Atlanta GA 30322 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenchun","family":"Chen","sequence":"additional","affiliation":[{"name":"Aflac Cancer and Blood Disorders Center Children's Healthcare of Atlanta Department of Pediatrics Emory University  Atlanta GA 30322 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Chemistry Emory University  Atlanta GA 30322 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxanne","family":"Glazier","sequence":"additional","affiliation":[{"name":"Wallace H. 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