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The triphosphates were good substrates for KOD XL DNA polymerase in primer extension synthesis of modified DNA. The nucleotide bearing linear 3,5\u2010dioxohexyl group (HDO) efficiently reacted with arginine\u2010containing peptides to form stable pyrimidine\u2010linked conjugates, whereas the branched 2\u2010acetyl\u20103\u2010oxo\u2010butyl (PDO) group was not reactive. Reaction with Lys or a terminal amino group formed enamine adducts that were prone to hydrolysis. 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