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Herein, we report a simple platform for DNA detection that takes advantage of four techniques: commercially available pregnancy test strips (PTS), amplicon generation via loop\u2010mediated isothermal amplification (LAMP), toehold\u2010mediated strand displacement, and noncovalent immobilization of DNA on paper surface with DNA nanoflowers. This simple, separation\u2010free platform is highly specific, as demonstrated with the detection of rtL180M, a single\u2010nucleotide polymorphism observed in hepatitis\u2005B virus (HBV) associated with antiviral drug resistance. It is very sensitive, capable of detecting the targeted mutation at 2\u2005copies\u2009\u03bcL<jats:sup>\u22121<\/jats:sup>. It is able to correctly identify the unmutated and rtL180M genome types of HBV in clinical samples. Given its wide adaptability, we expect this platform can be easily modified for the detection of genetic variations associated with various pathogens and human diseases.<\/jats:p>","DOI":"10.1002\/anie.202108827","type":"journal-article","created":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T14:20:10Z","timestamp":1629901210000},"page":"24823-24827","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["A DNA Nanoflower\u2010Assisted Separation\u2010Free Nucleic Acid Detection Platform with a Commercial Pregnancy Test Strip"],"prefix":"10.1002","volume":"60","author":[{"given":"Lijuan","family":"Qi","sequence":"first","affiliation":[{"name":"State key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences  Jilin P. R. China"},{"name":"Department of Chemistry University of Science and Technology of China  Anhui P. R. 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